pCMV-Red Firefly Luc Vector for Luciferase Assays
pCMV-Red Firefly Luc Vector for Luciferase Assays
Thermo Scientific™

pCMV-Red Firefly Luc Vector for Luciferase Assays

Thermo Scientific pCMV-Red Firefly Luc is a constitutive expression vector having the luciferase gene under the CMV (Cytomegalovirus) promoter forRead more
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Catalog number 16156
Price (USD)
120.00
Each
Add to cart
Price (USD)
120.00
Each
Add to cart
Thermo Scientific pCMV-Red Firefly Luc is a constitutive expression vector having the luciferase gene under the CMV (Cytomegalovirus) promoter for co-transfection and normalization control to account for experimental variation in combination with other reporters in a gene regulation study using the intracellular red firefly luciferase reporter with excellent light intensity..

Features of Red Firefly Luc:
• Red Firefly Luc Vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.
• pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
• pCMV and pTK vectors have the luciferase gene under the CMV promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively.
• These pCMV and pTK constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

These vectors are subject to a limited use label license.

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pMCS-Red Firefly Luc Vector for Luciferase Assays
pTK-Red Firefly Luc Vector for Luciferase Assays
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionpCMV-Red Firefly Luc Vector
TypeLuc Vector
Cloning MethodRestriction Enzyme/MCS
Delivery TypeTransfection
Quantity10 μg
Reporter GeneRed Firefly Luciferase
Selection Agent (Eukaryotic)Puromycin
Product LinePierce™
For Use With (Application)Reporter Assays
PromoterCMV
Unit SizeEach
Contents & Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.

Frequently asked questions (FAQs)

What options do you offer for luciferase vectors?

There are three vectors available for each expressed luciferase; pMCS, pCMV, and pTK. We recommend using pMCS to measure the activity of the reporter, and pCMV, and pTK as positive control vectors. For high level constitutive expression of Luciferase, we recommend using the CMV (Cytomegalovirus) promoter vector and for low level constitutive expression of luciferase, we recommend using the pTK (thymidine kinase) promoter vector.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the stability of the secreted luciferase?

Gaussia, Gaussia-Dura, and Cypridina Luciferase are stable in the culture media for greater than 24 hours. Generally, after transfection of CMV driven constructs, we see ever increasing secreted signal between 24 and 72 hours.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How quickly is luciferase secreted?

The earliest detectable signal will depend on the strength of the promoter. In general, with a strong promoter (such as CMV), significant signal over background can be seen in as little as 20 minutes. However, with a weaker inducible promoter, significant signal over background may take 1-2 hours after induction.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do you still offer quantitative IP (qIP) Luciferase assay kits?

Our qIP Luciferase assay kits have been discontinued but we do carry epitope-tagged (HA or c-Myc) and Tluc (TurboLuc luciferase)-tagged mammalian expression vectors, and qIP protein interaction assay reagents for these assay kits.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.