UltraPure™ Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications.Read more
Have Questions?
Change view
Catalog Number
Quantity
16520100
100 g
16520050
50 g
2 Options
Catalog number 16520100
Price (USD)
664.00
Each
Add to cart
Quantity:
100 g
Request bulk or custom format
Price (USD)
664.00
Each
Add to cart
UltraPure™ Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Low Melting Point Agarose is ideal for resolving DNA and RNA fragments and for the recovery of nucleic acid fragments after electrophoresis, as it melts at 65.5°C (below the melting point of most nucleic acids). The solution remains fluid at 37°C and will set rapidly at temperatures below 25°C. Benefits of using UltraPure™ Low Melting Point Agarose:
• Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Ideal for a variety of applications such as in-gel manipulation, tissue and cell culture, and viral plaque assays • Can also be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay)
Improved packaging The new environmentally friendly packaging uses 75% less plastic than the original bottles (see figure). This means less energy and raw material used in manufacture, and less waste in landfills. Additionally, the easy-pour spout reduces the likelihood of spills and contamination.
Performance and quality testing The performance of UltraPure™ Agarose is evaluated to satisfy specifications in appearance, moisture, gel strength, gelling temperature, melting temperature, sulfate content, electroendosmosis, and DNase/RNase assay.
Note: UltraPure™ Low Melting Point Agarose (Cat. No. 16520-100) is a replacement for the item previously sold under Cat. No. 15517-022.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeAgarose
Separation Range100 to >30000 bp
FormPowder
For Use With (Application)Nucleic Acid Gel Electrophoresis, Protein Electrophoresis, Blotting
Gel CompatibilityAgarose Gels
Green FeaturesSustainable packaging
Melting PointLow Melting Point
Product LineUltraPure™
Quantity100 g
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
1 pouch containing 100 g of UltraPure™ Low Melting Point Agarose. Store at room temperature.
Frequently asked questions (FAQs)
I want to pour my own gels. Which agarose should I use?
Our UltraPure Agarose is standard melting-point agarose designed for routine separation and analysis of DNA and RNA fragments in the 500-23,000 nt range. UltraPure Agarose 1000 is a specialized agarose that provides higher resolution of PCR fragments and other short DNA fragments. We also offer an UltraPure Low Melting Point Agarose, which is ideal for resolving DNA fragments from 10 to 1,000 bp with a low melting temperature of 65°C or less.
How can generation of replication competent adenoviruses be avoided when using your pSilencer adeno 1.0-CMV System?
Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.