Nunc™ Lab-Tek™ Chamber Slide System
Nunc™ Lab-Tek™ Chamber Slide System
Thermo Scientific™

Nunc™ Lab-Tek™ Chamber Slide System

Grow anchorage dependent cells right on the microscope slide using the unique removable chamber of the Thermo Scientific™ Nunc™ Lab-Tek™ Chamber Slide System.
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Catalog NumberWellsMaterialUnit Size
1773802Soda Lime GlassCase of 96
17108016GlassCase of 96
1773721Soda Lime GlassCase of 96
177372PK1Soda Lime GlassPack of 16
177380PK2Soda Lime GlassPack of 16
1773994Soda Lime GlassCase of 96
177399PK4Soda Lime GlassPack of 16
1774028Soda Lime GlassCase of 96
177402PK8Soda Lime GlassPack of 16
1774101Permanox PlasticCase of 96
177410PK
also known as 177410
1Permanox PlasticPack of 16
1774292Permanox PlasticCase of 96
177429PK
also known as 177429
2Permanox PlasticPack of 16
1774374Permanox PlasticCase of 96
177437PK4Permanox PlasticPack of 16
1774458Permanox PlasticCase of 96
177445PK8Permanox PlasticPack of 16
17859916Soda Lime GlassCase of 96
178599PK16Soda Lime GlassPack of 16
Catalog number 177380
Price (USD)
1,112.00
Case of 96
Add to cart
Wells:
2
Material:
Soda Lime Glass
Unit Size:
Case of 96
Price (USD)
1,112.00
Case of 96
Add to cart

Grow, stain, and examine cells directly on the Thermo Scientific™ Nunc™ Lab-Tek™ Chamber Slide™, which consists of a polystyrene media chamber attached to a microscopic slide. Slide surface is specially treated for adherent cell culture. Choose either specialty washed Soda Lime Glass slide or cell attachment optimized Permanox Plastic slide. Media chamber is easily removable without the need for a tool.

Features:
  • The solvent-resistant Permanox™ Plastic Chamber Slides are available in 1-, 2-, 4- and 8-well formats.
  • The specialty washed Soda Lime Glass Chamber Slides are available in 1-, 2-, 4-, 8- and 16-well formats.
  • Media chambers can be removed from slides without the need for a tool
  • Medical-grade non-toxic silicone sealing gasket can be removed with a pair of pointy tweezers before applying coverslip.
  • "Sand blasted" writing area provides easy identification of samples.
  • Coverglass 171080 is to be used with the 16-well glass Chamber Slide 178599.

  • Ordering Information:

    • Can be purchased in case of 96 or pack of 16.
    • Shipped sterile in convenient stackable incubation trays.
  • Warranty: 90 days
WARNING: Cancer – www.P65Warnings.ca.gov
Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.
Specifications
Wells2
Culture Area4.2 cm2
Lid MaterialPolystyrene
Description2-well Chamber Slide w/ removable wells
Surface TreatmentCell Culture
MaterialSoda Lime Glass
Volume (Metric) Working1.2 to 2.0 mL
IncludesClear polystyrene lid with handle on the side for aseptic manipulations
No. per Pack16
Dimensions (LxW)75 x 25 mm
SterilitySterile
Closure ColorClear
Unit SizeCase of 96

Frequently asked questions (FAQs)

What cell density should I use for Lab-Tek Chambered Slides and Lab-Tek II Chambered Slides?

Unfortunately, we do not have a recommended cell density for our LabTek Chamber Slides. However, you can refer to the following link, which provides recommended cell densities for our other cell culture products:
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/cell-culture-useful-numbers.html

What is the difference between your original Nunc Lab-Tek Chamber Slide System and your Nunc Lab-Tek II Chamber Slide System?

Please review the following key differences between the Nunc Lab-Tek Chamber Slide System and the Nunc Lab-Tek II Chamber Slide System:
- The Nunc Lab-Tek Chamber Slide System is available in Permanox plastic or glass and incorporates a silicone gasket.
- The Nunc Lab-Tek II Chamber Slide System is available in glass only and do not have a silicone gasket.
- The Nunc Lab-Tek II Chamber Slide System has a blue hydrophobic border and the upper structure is attached by an adhesive. Supplied with this product is a key and holder system which is used to remove the upper structure from the glass slide.
- The Nunc Lab-Tek Chamber Slide System is available in 1-, 2-, 4-, 8- or 16-well formats.
- The Nunc Lab-Tek II Chamber Slide System is available in 1-, 2-, 4- or 8-well formats.

Why do cells grow in lines when I am using glass Nunc Lab-Tek Chamber Slide products? How can I prevent this?

The following are possible causes and solutions:
- The cells could be stressed due to media substitution, especially from serum-free formulations, or other changes to the culture environment such as in temperature, humidity, and CO2 percentage. If possible, always plate cells in serum-containing medium. Switching cells to a serum-free media should be done gradually.
- The cells could be plated at very low densities. Most cell lines have a minimal platting density below which normal cell proliferation cannot occur, and therefore should be avoided.
- The cells could be poorly adherent. Treatment of cells that may reduce adhesion and therefore, should not be done immediately after plating, but after the cells have resumed exponential growth.

Can I fix and stain my Nunc Lab-Tek Chamber Slide products with the upper structure still attached?

The upper structure of our Nunc Lab-Tek Chamber Slide products are made of polystyrene so it is not compatible with many fixation reagents. These chamber slide products are designed for upper structure and gasket removal before fixing, staining, and coverslipping.

Can I use my Nunc Lab-Tek Chamber Slide products for fluorescence applications?

Our Nunc Lab-Tek Chamber Slide products are used for fluorescence analyses at the microscopic level.
The following are commonly used excitation and emittance wavelengths:
FITC
- Excitation: 420-490 nm
- Emittance: 520-550 nm
Rhodamine
- Excitation: 510-560 nm
- Emittance: 570-610 nm
Hoechst
- Excitation: 330-380 nm
- Emittance: 480-510 nm

View all Nunc™ Lab-Tek™ Chamber Slide System FAQs