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Bacterial Alkaline Phosphatase
Bacterial Alkaline Phosphatase (BAP) removes 3´ and 5´ phosphates from DNA and RNA. BAP is active at 65°C for atRead more
Catalog number 18011015
Price (USD)
366.00
Each
Price (USD)
366.00
Each
Bacterial Alkaline Phosphatase (BAP) removes 3´ and 5´ phosphates from DNA and RNA. BAP is active at 65°C for at least 1 h and is inactivated by phenol extraction.
Applications: Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation. Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.
Source: Purified from E. coli C90.
Performance and Quality Testing: Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; dephosphorylation
efficiency determined.
Unit Definition: One unit hydrolyzes 1 nmol of ATP in 30 min. at 37°C.
Unit Reaction Conditions: 10 mM Tris-HCl (pH 8.0), 1.5 mM [γ-32P]ATP, and enzyme in 50 μl for 30 min. at 37°C.
Applications: Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation. Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.
Source: Purified from E. coli C90.
Performance and Quality Testing: Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; dephosphorylation
efficiency determined.
Unit Definition: One unit hydrolyzes 1 nmol of ATP in 30 min. at 37°C.
Unit Reaction Conditions: 10 mM Tris-HCl (pH 8.0), 1.5 mM [γ-32P]ATP, and enzyme in 50 μl for 30 min. at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Shipping ConditionWet Ice
EnzymeBAP
Quantity2500 U
Product TypeAlkaline Phosphatase
Unit SizeEach
Contents & Storage
Bacterial Alkaline Phosphatase is supplied with a vial of 10X dephosphorylation buffer [100 mM Tris-HCl (pH 8.0)]. Store at -20°C.