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Ribonuclease H
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand of an RNA-DNA hybrid to produce 5´Read more
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| Catalog Number | Quantity |
|---|---|
| 18021071 | 120 U |
| 18021014 | 30 U |
Catalog number 18021071
Price (USD)
670.00
Each
Quantity:
120 U
Price (USD)
670.00
Each
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand of an RNA-DNA hybrid to produce 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA.
Applications
Removal of mRNA during second-strand cDNA synthesis. Removal of poly(A) sequences from mRNA in the presence of oligo(dT). Oligodeoxyribonucleotide-directed cleavage of RNA.
Source
Purified from E. coli expressing the E. coli RNase H gene on a plasmid.
Performance and quality testing
Ribonuclease, nonspecific endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease.
Unit definition
One unit hydrolyzes 1 nmol of RNA in 3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min at 37°C.
Unit reaction conditions
20 mM Tris-HCl (pH 7.5), 0.1 M KCl, 10 mM MgCl2 , 0.1 mM DTT, 5% (w/v) sucrose, 0.5 nmol 3H-labeled poly(A)poly(dT), and enzyme in 50 μL for 20 min at 37°C.
Applications
Removal of mRNA during second-strand cDNA synthesis. Removal of poly(A) sequences from mRNA in the presence of oligo(dT). Oligodeoxyribonucleotide-directed cleavage of RNA.
Source
Purified from E. coli expressing the E. coli RNase H gene on a plasmid.
Performance and quality testing
Ribonuclease, nonspecific endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease.
Unit definition
One unit hydrolyzes 1 nmol of RNA in 3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min at 37°C.
Unit reaction conditions
20 mM Tris-HCl (pH 7.5), 0.1 M KCl, 10 mM MgCl2 , 0.1 mM DTT, 5% (w/v) sucrose, 0.5 nmol 3H-labeled poly(A)poly(dT), and enzyme in 50 μL for 20 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration2 U/μL
Shipping ConditionApproved for shipment on Wet or Dry Ice
EnzymeRNase
Quantity120 U
Product TypeRibonuclease H
Unit SizeEach
Contents & Storage
4 vials of Ribonuclease H with each containing 30 units supplied at a concentration of 2U/μl
Store at -20°C in a non-frost-free freezer.
Store at -20°C in a non-frost-free freezer.