Lipofectamine™ Transfection Reagent
See the new Lipofectamine® Transfection Reagent protocol—now optimized for efficiency, viability and reproducibility
Lipofectamine™ Transfection Reagent
Invitrogen™

Lipofectamine™ Transfection Reagent

Lipofectamine™ Transfection Reagent is one of our first-generation, cationic-lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells.Read more
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Catalog NumberQuantity
183240121 mL
183240100.3 mL
183240204 mL
Catalog number 18324012
Price (USD)
642.00
Each
Add to cart
Quantity:
1 mL
Price (USD)
642.00
Each
Add to cart

Lipofectamine™ Transfection Reagent is one of our first-generation, cationic-lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells. Lipofectamine™ Transfection Reagent is a trusted, value reagent with many years of cited use in thousands of publications and with many cell lines.

With Lipofectamine™ Transfection Reagent, you'll get:

  • A reagent proven to work in high-throughput applications
  • A reliable reagent for establishing stable cell lines
  • A reagent that works well with PLUS™ reagent for higher protein expression

A proven technology

Our Lipofectamine™-brand reagents have been recognized as the most-cited transfection reagent family, with tens of thousands of citations since launch in 1993. Lipofectamine™ Transfection Reagent is no exception as a trusted reagent for a wide range of cell lines. When used in combination with PLUS™ Reagent, Lipofectamine™ Transfection Reagent has been shown to work well with cells such as BHK-21, NIH 3T3, COS-1, fibroblasts, keratinocytes, HT-29, MRC-5, and SK-BR3.

A 'value' alternative for plasmid DNA

With such a legacy comes a commitment to providing the best reagents for all scientists and labs, regardless of their funding capabilities. Lipofectamine™ Transfection Reagent is a value alternative for transfection of plasmid DNA into eukaryotic cells with a simplified protocol. For optimal results in a wider range of plasmid DNA transfection conditions, we recommend Lipofectamine™ LTX Reagent, due to its optimal balance of potency & low-cytotoxicity. Lipofectamine™ 2000 Transfection Reagent is recommended if you are transfecting a broad range of cell lines or want to transfect RNA.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEstablished Cell Lines, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity1 mL
Serum CompatibleNo
Shipping ConditionWet Ice
Unit SizeEach
Contents & Storage
Each unit contains one or multiple vials of reagent. Store at 2°C to 8°C.

Frequently asked questions (FAQs)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

Is it necessary to use serum-free medium during lipid transfection?

It is not necessary to use serum-free medium during lipid transfection. However, it is critical to form the lipid:nucleic acid complex in the absence of serum, because proteins can interfere with complex formation. Once the complexes are formed, they can be added to cells in serum-containing medium. For optimal results with Lipofectin Transfection Reagent, we recommend performing transfection in medium without serum.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

View all Lipofectamine™ Transfection Reagent FAQs