Oligo(dT)12-18 Primer
Oligo(dT)<sub>12-18</sub> Primer
Invitrogen™

Oligo(dT)12-18 Primer

Oligo(dT)12-18 Primer is suitable for use in first-strand cDNA synthesis with reverse transcriptase. The primer hybridizes to the poly(A) tailRead more
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Catalog number 18418012
Price (USD)
111.00
Each
Add to cart
Price (USD)
111.00
Each
Add to cart

Oligo(dT)12-18 Primer is suitable for use in first-strand cDNA synthesis with reverse transcriptase. The primer hybridizes to the poly(A) tail of mRNA. It is phosphorylated on the 5´ end to facilitate cloning of cDNA.

Performance and quality testing: Performance is evaluated in a first-strand cDNA synthesis reaction

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypePrimer
5'Primer ModificationPhosphate
Primer Sequence5'd PO4 [(T)12-18] 3'
Purification MethodGel-purified, Desalted
Shipping ConditionApproved for shipment on Wet or Dry Ice
Concentration0.5 μg/μL
PrimerOligo dT
Quantity50 μL
For Use With (Application)First-strand cDNA Synthesis
FormLiquid
Unit SizeEach
Contents & Storage
• Oligo(dT)12-18 Primer in DEPC-treated water (50 μg at 0.5 μg/μL)

Store at –20°C.
Guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

How does the Anchored Oligo(dT)20 Primer differ from standard oligo(dT) primers?

Anchored Oligo(dT) primers have 2 random bases at the 3' end of the stretch of Ts. The first random base is either an A, G, or C, while the second can be any of the 4 standard nucleotides, i.e., A, T, G or C. The use of anchored oligo(dT) primers results in increased cDNA synthesis yields.

For first-strand cDNA synthesis, is it better to use oligo(dT), random hexamers, gene-specific primer (GSP), or combination of these primers?

The choice of primer depends on your experimental goals. Oligo(dT) is recommended when using total RNA for cDNA synthesis. It is the key to full-length cDNA synthesis. Random hexamers give a series of short first-strand products spanning the entire mRNA. Use of random hexamers may be helpful if the PCR fragment is at the 5´ end of a large mRNA. To ensure full-length cDNA synthesis of large transcripts, oligo(dT) can be added along with random hexamers during first-strand synthesis. Gene-specific primers (GSP) for cDNA synthesis may also be used and are required in a few applications such as 5´ RACE and qRT-PCR. For GC-rich templates or templates rich in secondary structure, a GSP may not work as well as priming with oligo dT for first-strand synthesis. If an RT-PCR is problematic, trying different options of oligo dT, random primers and/or GSP for priming first-strand synthesis may resolve the issue. Oligo(dT)20 primer (Cat. No. 18418-020) is recommended for use with SuperScript III Reverse Transcriptase (Cat. no. 18080-044), ThermoScript Reverse Transcriptase (Cat. No. 12236-014), Thermo-X Reverse Trascriptase (Cat. No. 11150-025), and Cloned AMV Reverse Transcriptase (Cat. No. 12328-019).

Reference:
Frohman,M.A., Dush,M.K., Martin, G.R. (1988) Proc. Nat. Acad. Sci USA 85, 8998