dNTP Mix (10 mM ea)
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dNTP Mix (10 mM ea)
Invitrogen™

dNTP Mix (10 mM ea)

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is atRead more
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Catalog NumberQuantity
184270881 mL
184270895 x 1 mL
18427013100 μL
Catalog number 18427088
Price (USD)
698.00
Each
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Quantity:
1 mL
Request bulk or custom format
Price (USD)
698.00
Each
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Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes including: polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Features include:
• Chemically synthesized
• >99% purity confirmed by HPLC
• Stable for 2 years at –20°C
• Free from qPCR, PCR, reverse transcription inhibitors
• Free of DNases and RNases
• Free of human and E. coli DNA

Applications
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA synthesis
• High-fidelity and long-range PCR
• Isothermal amplification
• DNA labeling
• Cloning
• Sanger and next-generation sequencing (NGS)

Thermo Fisher Scientific offers a wide variety of dNTPs in different concentrations, volumes, and formats.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormLiquid
Label or DyeUnlabeled
Quantity1 mL
Shipping ConditionApproved for shipment on Wet or Dry Ice
For Use With (Application)Standard PCR, RT-PCR, Real Time PCR (qPCR)
Concentration10 mM
Unit SizeEach
Contents & Storage
dNTP Mix, contains 10 mM each of dATP, dCTP, dGTP, and dTTP (1 mL)

Store at –20°C.

Frequently asked questions (FAQs)

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

Why do some dNTP mixes contain dTTP and others TTP?

Thymine is normally found as a deoxyribose. Thus, TTP and dTTP are essentially the same thing. Both types of mixes can be used in PCR reactions

What concentration of dNTPs is typically used in a PCR amplification?

The four dNTPs (dATP, dCTP, dTTP, dGTP) are used at a final concentration of 200 µM each or a total of 0.8 mM in standard amplifications. For PCR amplifications that require high fidelity, lower dNTP concentrations (on the order of 10-50 µM) work optimally.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.