BLOCK-iT™ Fluorescent Oligo, for lipid transfection
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Invitrogen™

BLOCK-iT™ Fluorescent Oligo, for lipid transfection

BLOCK-iT™ Fluorescent Oligo provides:• Strong, easily detectable FITC signal indicating transfection efficiency• Chemical modifications for a clearer, more persistent cellularRead more
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Catalog number 2013
Price (USD)
576.00
Each
Add to cart
Price (USD)
576.00
Each
Add to cart
BLOCK-iT™ Fluorescent Oligo provides:

• Strong, easily detectable FITC signal indicating transfection efficiency
• Chemical modifications for a clearer, more persistent cellular signal than labeled siRNA
• Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA molecules

This makes the BLOCK-iT™ Fluorescent Oligo an ideal indicator of transfection efficiency for RNAi experiments. BLOCK-iT™ Fluorescent Oligo is available in two configurations: one designed for lipid-mediated transfection (2 x 125 μL) at 20 μM and one designed for electroporation (75 μl) at 1 mM.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeOligonucleotides
Label or DyeFITC (Fluorescein)
Product LineBLOCK-iT™
For Use With (Application)Lipid Transfection
Unit SizeEach
Contents & Storage
The BLOCK-iT™ Fluorescent Oligo for lipid transfection is supplied as 2 x 125 μl tubes at 20 μM. Stored at -20°C.

Frequently asked questions (FAQs)

What are the positive and negative controls I should use with my Stealth RNAi siRNA experiment?

We offer several negative and positive controls for Stealth siRNA:

For Stealth Positive Control:
- Stealth RNAi siRNA GAPDH Positive Control (human) (Cat. No. 12935140, 250 µL)
- Stealth RNAi siRNA beta-Lactamase Reporter Control (Cat. No. 12935148, 250 µL)
- Stealth RNAi siRNA GFP Reporter Control (Cat. No. 12935145, 250 µL)
- Stealth RNAi siRNA Luciferase Reporter Control (Cat. No. 12935146, 250 µL)

For Stealth Negative Control:
Hi (55-65% GC), Med (45-55% GC), or Low (35-45% GC) Duplexes are offered:

- Stealth RNAi siRNA Negative Control Hi GC (Cat. No. 12935400, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #2 (Cat. No. 12935114, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935115, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex (Cat. No. 12935300, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex #2 (Cat. No. 12935112, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935113, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex (Cat. No. 12935200, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #2 (Cat. No. 12935110, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #3 (Cat. No. 12935111, 250 µL)
- Stealth RNAi siRNA Negative Control Kit (Cat. No. 12935100, contains one of each GC content level negative control, 250 µL each at 20 µM)
- For a scrambled negative control, you could also scramble your designed siRNA or Stealth RNAi sequence using the BLOCK-iT RNAi Designer.

You can also use our BLOCK-iT AlexaFluor Red Fluorescent Control (Cat. No. 14750100) or BLOCK-iT Fluorescent Oligo (Cat. No. 2013, 13750062) as a control for the transfection.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Can I use the BLOCK-iT Fluorescent Oligo, for lipid transfection (Cat. No. 2013) to assess transfection efficiency with Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778100)?

  • We do not recommend using BLOCK-iT Fluorescent Oligo, for lipid transfection (Cat. No. 2013) to assess transfection efficiency with Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778100). BLOCK-iT Alexa Fluor Red Fluorescent Control (Cat. No. 14750100) should be used to assess transfection efficiency with Lipofectamine RNAiMAX Reagent.
  • BLOCK-iT Fluorescent Oligo, for lipid transfection is optimized for use with Lipofectamine 2000 Transfection Reagent and is not recommended for Lipofectamine RNAiMAX Reagent.


    • Find additional tips, troubleshooting help, and resources within our RNAi Support Center