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Pierce™ Coomassie Brilliant Blue Dyes
Thermo Scientific Pierce Coomassie Brilliant Blue dyes are composed of one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains.
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| Catalog Number | Description |
|---|---|
| 20278 | Pierce Coomassie Brilliant Blue R-250 Dye |
| 20279 | Pierce Coomassie Brilliant Blue G-250 Dye |
Catalog number 20278
Price (USD)
182.00
Each
Description:
Pierce Coomassie Brilliant Blue R-250 Dye
Price (USD)
182.00
Each
Thermo Scientific Pierce Coomassie Brilliant Blue dyes are composed of one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains. Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye.
Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye.
Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol. In acidic conditions, the dye binds to proteins primarily through basic amino acids (primarily arginine, lysine, and histidine), and the number of coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Protein binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm).
Features include:
• Easy detection—develops intensely colored complexes with proteins
• High sensitivity—can determine as little as 0.5 μg/cm2 of protein present in a gel matrix
• Reversible staining—anion of Coomassie Brilliant Blue dye formed in the acidic staining medium combines with the protonated amino groups of proteins by electrostatic interaction; resulting complex is reversible under proper conditions
• Differentiation between bound and unbound dye—when dissolved in 0.01 M citrate buffer at pH 3.0, has an absorption maximum at 555 nm; protein-dye complex is characterized by a peak slightly broader than that of free dye with a maximum at 549 nm
Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol. In acidic conditions, the dye binds to proteins primarily through basic amino acids (primarily arginine, lysine, and histidine), and the number of coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Protein binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm).
Features include:
• Easy detection—develops intensely colored complexes with proteins
• High sensitivity—can determine as little as 0.5 μg/cm2 of protein present in a gel matrix
• Reversible staining—anion of Coomassie Brilliant Blue dye formed in the acidic staining medium combines with the protonated amino groups of proteins by electrostatic interaction; resulting complex is reversible under proper conditions
• Differentiation between bound and unbound dye—when dissolved in 0.01 M citrate buffer at pH 3.0, has an absorption maximum at 555 nm; protein-dye complex is characterized by a peak slightly broader than that of free dye with a maximum at 549 nm
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPierce Coomassie Brilliant Blue R-250 Dye
Detection LocationIn-Gel Detection, In-Solution Detection, In-Blot Detection
Detection MethodColorimetric
Label or DyeCoomassie
Product TypeProtein Gel Stain
Quantity50 g
Target MoleculeProtein
Unit SizeEach
Contents & Storage
Store at room temperature.