Detoxi-Gel™ Endotoxin Removing Gel
An new endotoxin detection kit is now available—Pierce Chromogenic Endotoxin Quant Kit, with higher sensitivity and no interference from β-glucans. To learn more about these new kits, please visit thermofisher.com/endotoxin.
Detoxi-Gel™ Endotoxin Removing Gel
Thermo Scientific™

Detoxi-Gel™ Endotoxin Removing Gel

Thermo Scientific Detoxi-Gel Endotoxin Removing Gel uses Polymixin B ligand immobilized on beaded affinity resin to bind and extract endotoxinsRead more
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Catalog NumberDescriptionQuantity
20339Detoxi-Gel Endotoxin Removing Gel10 mL
20340Detoxi-Gel Endotoxin Removing Gel1000 mL
Catalog number 20339
Price (USD)
260.00
Each
Add to cart
Description:
Detoxi-Gel Endotoxin Removing Gel
Quantity:
10 mL
Request bulk or custom format
Price (USD)
260.00
Each
Add to cart
Thermo Scientific Detoxi-Gel Endotoxin Removing Gel uses Polymixin B ligand immobilized on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.

Detoxi-Gel Endotoxin Removing Gel and quickly remove endotoxins (lipopolysaccharides or LPS) from samples. Even low levels of endotoxins can be toxic to cells or organisms and must be removed before biological samples can be introduced.

Features of Detoxi-Gel Endotoxin Removing Gel:

• Resin uses immobilized polymixin B to remove pyrogens by binding their lipid A domains
• Reduces endotoxin levels in protein solutions, cell culture media, solutions containing pharmacological components and aqueous buffers
• 1 mL of gel removes >9995 EU from a 10,000 EU challenge of LPS (greater than 99% efficiency)
• 1 mL of gel binds 4000-6000 E.U. of the lipopolysaccharide from E. coli strain 055:BS
• Protein recovery dependent upon protein type and concentration; some empirical testing required
• Stable and reusable; resin can be regenerated by stripping off the endotoxins with a 1% deoxycholate solution in pyrogen-poor water; no loss in binding capacity even after 10 regenerations

Related Products
Detoxi-Gel™ Endotoxin Removing Columns, 1 mL
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Capacity (Metric)4000 to 6000 EU Binding per mL Gel
DescriptionDetoxi-Gel Endotoxin Removing Gel
FormLiquid
Product LineDetoxi-Gel™
Quantity10 mL
TargetEndotoxin Removal
TypeEndotoxin Removing Gel
Stationary PhasePolymixin B
Sufficient For1 mL of gel binds 4000-6000 EU
FormatBottle
Column TypeAffinity, Agarose Resin
Unit SizeEach
Contents & Storage
Store at 4°C.

Frequently asked questions (FAQs)

Why must I regenerate the Detoxi-Gel Endotoxin Removing Gel column before and after each use?

It is possible that the column may have been contaminated with endotoxin during storage and handling. Equilibrating the column with 1% sodium deoxycholate followed by pyrogen-free washing ensures that the column is free of endotoxin before the sample is added. The column must be regenerated following endotoxin removal from a sample to make sure the endotoxin that has bound to the column from the sample is washed off.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the void volume of the Detoxi-Gel Endotoxin Removing Gel column?

The Thermo Scientific 1 mL Detoxi-Gel Column has a void volume of 0.3-0.5 mL. This is the volume of solution that must be collected from the column before the sample will begin to come off the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the protocol for using Detoxi-Gel Endotoxin Removing Gel?

1. Equilibrate the column with 5 column volumes of 1% sodium deoxycholate in pyrogen-free water, followed by 3 column volumes of pyrogen-free water or buffer of choice.
2. Apply sample, being sure not to exceed the binding capacity of the gel.
3. Collect sample by gravity flow by applying additional pyrogen-free water or buffer.
4. Regenerate the column with 5 column volumes of 1% sodium deoxycholate in pyrogen-free water, followed by 3 column volumes of pyrogen-free water.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the binding capacity of Detoxi-Gel Endotoxin Removing Gel?

Detoxi-Gel Support has the capacity to bind up to 2µg LPS/mL of gel. Because one endotoxin unit (EU) is approximately 0.1 ng/mL, the gel has the potential for binding up to 20,000 EU/mL of gel.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is Detoxi-Gel Endotoxin Removing Gel, and how does it work?

Detoxi-Gel Support consists of 6% cross-linked beaded agarose that has Polymixin B immobilized onto it. Polymixin B is an antibiotic that contains a cationic cyclopeptide with a fatty acid chain that can neutralize the biological activity of endotoxins by binding to the lipid A portion of the bacterial lipopolysacharide (LPS). After the endotoxin from a sample binds to the immobilized Polymixin B, the sample can be collected and the endotoxin can be eluted using 1% sodium deoxycholate.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.