Thermo Scientific Pierce Streptavidin is a purified unconjugated recombinant protein form.Features of Streptavidin:• Recombinant streptavidin—high-quality protein, 53kDa, pI = 6.8Read more
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Catalog Number
Quantity
21122
1 mg
21125
5 mg
21135
100 mg
21145
1000 mg
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Catalog number 21122
Price (USD)
186.00
Each
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Quantity:
1 mg
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Price (USD)
186.00
Each
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Thermo Scientific Pierce Streptavidin is a purified unconjugated recombinant protein form.
Features of Streptavidin:
• Recombinant streptavidin—high-quality protein, 53kDa, pI = 6.8 to 7.5 • Pure protein —choose pure, unconjugated protein for biotin blocking and controls
The purified recombinant Streptavidin enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin is supplied as lyophilized in phosphate-based buffers for immediate reconstitution with water.
Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Thermo Scientific Pierce Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ConjugateUnconjugated
Quantity1 mg
FormSolid
Product LinePierce™
Product TypeStreptavidin
Target MoleculeBiotin
Label or DyeUnlabeled
Unit SizeEach
Contents & Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Frequently asked questions (FAQs)
How many biotin binding-sites are there per streptavidin molecule?
Streptavidin is a protein composed of four identical subunits, each containing a high affinity binding site for biotin (K-D = 10 -15 M) . Streptavidin has the same biotin binding properties as avidin, but it has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low non-specific binding.