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Pierce™ His Protein Interaction Pull-Down Kit
Thermo Fisher Scientific Pierce His Tag Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins thatRead more
Catalog number 21277
Price (USD)
550.00
Each
Price (USD)
550.00
Each
Thermo Fisher Scientific Pierce His Tag Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with His-tagged fusion proteins.
Features of His Tag Protein Interaction Pull-Down Kit:
• 6xHis pull-down (Product No. 21277)—purifies protein interactors of any His-tagged fusion protein
• Complete kits—provide all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)
• Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
• Flexible—instructions include protocols for use with bait and prey proteins expressed from a variety of sample types
• Less non-specific binding—Cobalt chelate resin is more specific for histidine-tagged fusion proteins than nickel resins, resulting in less non-specific binding
• Binding—Binds 10 to 25 mg of histidine-tagged fusion protein per mL of resin
Applications:
• Discover a new protein:protein interaction from a cell lysate
• Confirm a putative interaction from a cell lysate or with a previously purified protein
• Extract protein:protein interaction information from in vitro transcription/translation lysates
You provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.
Related Products
Pierce™ GST Protein Interaction Pull-Down Kit
Features of His Tag Protein Interaction Pull-Down Kit:
• 6xHis pull-down (Product No. 21277)—purifies protein interactors of any His-tagged fusion protein
• Complete kits—provide all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)
• Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
• Flexible—instructions include protocols for use with bait and prey proteins expressed from a variety of sample types
• Less non-specific binding—Cobalt chelate resin is more specific for histidine-tagged fusion proteins than nickel resins, resulting in less non-specific binding
• Binding—Binds 10 to 25 mg of histidine-tagged fusion protein per mL of resin
Applications:
• Discover a new protein:protein interaction from a cell lysate
• Confirm a putative interaction from a cell lysate or with a previously purified protein
• Extract protein:protein interaction information from in vitro transcription/translation lysates
You provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.
Related Products
Pierce™ GST Protein Interaction Pull-Down Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayAffinity Purification
For Use With (Equipment)Microcentrifuge
Product LinePierce™
Quantity1 Kit
For Use With (Application)Pull-Down (Tag-Based)
TargetNot Target-Specific
TypePull-Down Kit
Sufficient For25 Reactions
FormatKit
Unit SizeEach
Contents & Storage
Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin
• Cobalt Chelate Agarose Resin, 750 μL
• Pull-Down Lysis Buffer, 250 mL
• Imidazole Solution (4M), 5 mL
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes
Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
• Cobalt Chelate Agarose Resin, 750 μL
• Pull-Down Lysis Buffer, 250 mL
• Imidazole Solution (4M), 5 mL
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes
Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.