Fischer's Medium
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Gibco™

Fischer's Medium

Fischer's medium was originally developed by Glen Fischer at the National Cancer Institute (Bethesda, MD) and formulated for the cultureRead more
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Catalog number 21475025
Price (USD)
81.75
Each
Add to cart
Price (USD)
81.75
Each
Add to cart

Fischer's medium was originally developed by Glen Fischer at the National Cancer Institute (Bethesda, MD) and formulated for the culture of mouse leukemic cells. Fischer's Medium is primarily used for the culture of hematopeitic cells, such as lymphoblasts and lymphocytes.

This Fischer's medium is manufactured as follows:

WithWithout
• L-glutamine• HEPES
• Phenol Red 


The complete formulation is available.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

Fischer's medium contains no proteins, lipids, or growth factors. Fischer's medium typically requires supplementation of Fetal Bovine Serum (FBS). Fischer's medium uses a sodium bicarbonate buffer system (1.125 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
Concentration1 X
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeFischer's Medium
Shelf Life12 Months
SterilitySterile-filtered
With AdditivesGlutamine, Phenol Red
Without AdditivesNo HEPES
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fischer's Medium FAQs