Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can beRead more
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Catalog number 21565
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Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.
Features of the EGS crosslinker:
• Reactive groups:NHS ester (both ends) • Reactive towards: amino groups (primary amines) • Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS • Membrane-permeable, allowing for intracellular crosslinking • Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C • Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS
Product References: Crosslinker Application Guide -- search for recent literature references for this product