EGS (ethylene glycol bis(succinimidyl succinate))
EGS (ethylene glycol bis(succinimidyl succinate))
Thermo Scientific™

EGS (ethylene glycol bis(succinimidyl succinate))

Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can beRead more
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Catalog number 21565
Price (USD)
250.00
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Price (USD)
250.00
Each
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Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Features of the EGS crosslinker:

Reactive groups: NHS ester (both ends)
Reactive towards: amino groups (primary amines)
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
• Membrane-permeable, allowing for intracellular crosslinking
• Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

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EGS (ethylene glycol bis(succinimidyl succinate))
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine-Amine
CleavableBy Hydroxylamine
DescriptionEGS
Molecular Weight (g/mol)456.36
PEGylatedNo
Spacer Arm Length16.1 Å
Cell PermeabilityYes
Shipping ConditionAmbient
Product LinePierce™
Labeling MethodChemical Labeling
Crosslinker TypeHomobifunctional
Reactive MoietyNHS Ester
SpacerMedium (10 to 30 Å)
FormPowder
Quantity1 g
SolubilityDMF, DMSO
FormatStandard
Water SolubleNo
Product TypeCrosslinker
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.