PDPH (3-(2-pyridyldithio)propionyl hydrazide)
PDPH (3-(2-pyridyldithio)propionyl hydrazide)
Thermo Scientific™

PDPH (3-(2-pyridyldithio)propionyl hydrazide)

Thermo Scientific Pierce PDPH is a short, pyridyldithiol-and-hydrazide crosslinker for reversibly conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, suchRead more
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Catalog number 22301
Price (USD)
254.00
Each
Add to cart
Price (USD)
254.00
Each
Add to cart
Thermo Scientific Pierce PDPH is a short, pyridyldithiol-and-hydrazide crosslinker for reversibly conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Features of PDPH :

• Reactive groups: pyridyldisulfide and hydrazide
• Reactive toward: sulfhydryl groups and carbonyl (aldehyde) groups
• Short (9.2A), sulfhydryl-to-aldehyde crosslinker with disulfide bond spacer arm (cleavable)
• Pyridyldithiol group results in attachment to sulfhydryls via disulfide bond, which can be cleaved with DTT, TCEP or other reducing agents
• Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
• Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
• Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups

Product References:
Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityCarbohydrate-Sulfhydryl
CleavableBy Thiols
DescriptionPDPH
Molecular Weight (g/mol)229.32
PEGylatedNo
Spacer Arm Length9.2 Å
Cell PermeabilityYes
Shipping ConditionAmbient
Product LinePierce™
Labeling MethodChemical Labeling
Crosslinker TypeHeterobifunctional
Reactive MoietyHydrazide, Pyridyldisulfide
SpacerShort (<10 Å)
FormPowder
Quantity50 mg
SolubilityDMF, DMSO
FormatStandard
Water SolubleNo
Product TypeCrosslinker
Unit SizeEach
Contents & Storage
Upon receipt store desiccated at 4°C.

Frequently asked questions (FAQs)

Can you provide the shelf-life for PDPH (3-(2-pyridyldithio)propionyl hydrazide)?

PDPH (3-(2-pyridyldithio)propionyl hydrazide) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

We are using EDC to crosslink a protein onto carboxyl-modified beads. We would like to add in a linker molecule to extend the radius of beads to increase the surface area so more proteins can be immobilized. Is this possible?

To provide a linker between the beads and the target protein, you would need a molecule with a primary amine (to conjugate to the carboxyl group on the beads with EDC) on one end and another reactive group on the other (to conjugate to the protein). For instance, sulfo-SMCC (Cat. No. A39268) could be used in conjunction with PDPH (Cat. No. 22301):
The method is outlined below:
1. Conjugate the target protein to the NHS ester (amine-reactive) group of Sulfo-SMCC. This will produce a maleimide-activated target protein via its primary amines (lysines and N-terminus).
2. Remove the excess sulfo-SMCC by desalting or dialysis.
3. React the maleimide-activated target protein to PDPH via sulfhydryls. (Lysines and N-terminus now are connected to hydrazide (amine) group with an extended linker)
4. Remove excess PDPH by desalting or dialysis.
5. React the hydrazide-activated target protein with carboxyl beads in the presence of EDC.
6. Use appropriate buffer to rinse off excess target protein from the carboxyl beads.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.