Sulfo-EMCS (N-ε-maleimidocaproyl-oxysulfosuccinimide ester)

Thermo Scientific Pierce Sulfo-EMCS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).

Features of Sulfo-EMCS:

• Reactive groups: sulfo-NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• Sulfo-NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
• Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Non-cleavable
• Water-soluble (compare to EMCS)
• Aliphatic spacer offers low potential for eliciting an immune response

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)