Laminin Mouse Protein, Natural
Laminin Mouse Protein, Natural
Gibco™

Laminin Mouse Protein, Natural

Laminin is the major glycoprotein component of basement membranes, and has a molecular weight of 850,000 daltons. This preparation ofRead more
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Catalog number 23017015
Price (USD)
264.00
Each
Add to cart
Price (USD)
264.00
Each
Add to cart
Laminin is the major glycoprotein component of basement membranes, and has a molecular weight of 850,000 daltons. This preparation of mouse Laminin was isolated from the Engelbreth-Holm-Swarm (EHS) sarcoma and is supplied at a concentration of 0.5-2.0 mg⁄ml.

Key functions include:

• Modulation of cell attachment, cell spreading, cell growth and motility
• Binding to itself and other matrix compounds
• Promotion of epithelial differentiation
• Modification of leukocyte function
• Stimulation of neurite outgrowth

Supplied: 1 mg in 50 mM Tris-HCL (pH 7.4), 0.15 M NaCl

Performance qualification (cell attachment): Half-maximal cell attachment of MG63 cells at 37°C: ≤ 20 ug⁄ml
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Cell Culture
Quantity1 mg
Gene AliasLaminin
SpeciesMouse
Shipping ConditionDry Ice
FormLiquid
Protein SubtypeLaminin
Research CategoryExtracellular Matrix & Cell Adhesion, Stem Cell Research
Unit SizeEach
Contents & Storage
Store in ultra-cold freezer (-68 to -85°C) and protect from light.

Frequently asked questions (FAQs)

What is the molecular weight of Laminin Mouse Protein, Natural?

It has 3 subunits and has a MW of 850 kDa: one A chain is 400 kDa and two B chains are 205 and 215 kDa.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I would like to know how Laminin Mouse Protein, Natural was sterilized.

Laminin Mouse Protein, Natural (Cat. No. 23017015) is sterile filtered through a 0.22 micron filter membrane.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use the Poly-D-Lysine matrix with other coatings?

iPSC-derived neurons have been cultured successfully on Poly-D-Lysine with a secondary coating of mouse and human laminin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can natural mouse laminin be thawed, aliquoted, and refrozen?

Yes, it is recommended to store usage size aliquots at -68 degrees C to -85 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the protein concentration per vial for natural mouse laminin?

The protein concentration per vial is 1 mg/vial. The specification for protein concentration can range from 0.5 to 2.0 mg/mL. The volume per vial can vary as it will be based on the protein concentration determined for the specific lot. The volume added to each vial will equal 1 mg of laminin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.