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Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible
This BCA Protein Assay Kit is a reducing agent-compatible version of our popular Pierce BCA Protein Assay. The kit enablesRead more
Catalog number 23250
Price (USD)
330.00
Each
Price (USD)
330.00
Each
This BCA Protein Assay Kit is a reducing agent-compatible version of our popular Pierce BCA Protein Assay. The kit enables you to measure protein concentration in samples that contain the disulfide reducing agents.
Compare all available BCA protein assays ›
Features of the BCA Protein Assay Kit—Reducing Agent Compatible include:
• Compatibility—assay samples that contain up to 5 mM DTT, 35 mM BME, or 10 mM TCEP
• BCA technology—only a slight modification of the standard BCA Protein Assay protocol (15-min incubation with Compatibility Reagent); no precipitation steps required
• Sample volume—requires only 25 μL (standard kit) or less than 10 μL (microplate kit) of sample
• Colorimetric—measure with a standard spectrophotometer or plate reader at 562 nm
• Excellent uniformity—exhibits less protein-to-protein variation than dye-binding methods
• Assay range—linear working range for BSA of 125 to 2000 μg/mL
The BCA Protein Assay Kit—Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, BME, or TCEP. This reducing agent-compatible BCA kit extends the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.
Related products
Pierce Microplate BCA Protein Assay Kit - Reducing Agent Compatible
96-Well Plates for Pierce BCA-RAC Assay
Compare all available BCA protein assays ›
Features of the BCA Protein Assay Kit—Reducing Agent Compatible include:
• Compatibility—assay samples that contain up to 5 mM DTT, 35 mM BME, or 10 mM TCEP
• BCA technology—only a slight modification of the standard BCA Protein Assay protocol (15-min incubation with Compatibility Reagent); no precipitation steps required
• Sample volume—requires only 25 μL (standard kit) or less than 10 μL (microplate kit) of sample
• Colorimetric—measure with a standard spectrophotometer or plate reader at 562 nm
• Excellent uniformity—exhibits less protein-to-protein variation than dye-binding methods
• Assay range—linear working range for BSA of 125 to 2000 μg/mL
The BCA Protein Assay Kit—Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, BME, or TCEP. This reducing agent-compatible BCA kit extends the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.
Related products
Pierce Microplate BCA Protein Assay Kit - Reducing Agent Compatible
96-Well Plates for Pierce BCA-RAC Assay
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPierce BCA Tube Protein Assay Kit, Reducing Agent Compatible (RAC)
AssayBCA Assay
Detection MethodColorimetric
Product LinePierce™
Product TypeProtein Quantitation Assay
SpecificityNot Target-Specific
Sufficient For250 Tube Assays
For Use With (Application)Solution-based Detection, Absorbance
For Use With (Equipment)Spectrophotometer, Microplate Reader
Quantity275 mL
Unit SizeEach
Contents & Storage
Sufficient For: 250 tube assays with 25 μL samples
• BCA Reagent A, 250 mL
• BCA Reagent B, 25 mL
• Compatibility Reagent, 10 x 20 mg
• Reconstitution Buffer, 15 mL
• Albumin Standard Ampules, 2mg/mL, 10 x 1 mL
Store at room temperature
• BCA Reagent A, 250 mL
• BCA Reagent B, 25 mL
• Compatibility Reagent, 10 x 20 mg
• Reconstitution Buffer, 15 mL
• Albumin Standard Ampules, 2mg/mL, 10 x 1 mL
Store at room temperature