Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Thermo Scientific™

Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format

Thermo Scientific Pierce Sulfo-NHS, No-Weigh Format is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters forRead more
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Catalog NumberQuantity
245255 g
24510500 mg
A3926910 x 2 mg
Catalog number 24525
Price (USD)
1,894.00
Each
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Quantity:
5 g
Request bulk or custom format
Price (USD)
1,894.00
Each
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Thermo Scientific Pierce Sulfo-NHS, No-Weigh Format is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.

Features of Sulfo-NHS:

• Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS
• Amine-reactive NHS esters or Sulfo-NHS esters can be made with any carboxyl-containing molecule
• Sulfo-NHS derivatives are usually directly water-soluble (can be added directly to physiologic buffers) and membrane-impermable (can be used for cell surface labeling)
• High-purity, crystalline Sulfo-NHS can be used to create high-quality activated derivatives

Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by mixing the Sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.

Applications:
• Improve efficiency of EDC coupling reactions
• Convert carboxyls to amine-reactive Sulfo-NHS esters
• Crosslink proteins to carboxyl-coated beads or surfaces more efficiently
• Activate nanoparticles with amine-reactive Sulfo-NHS esters

Specifications for Sulfo-NHS:
We manufacture N-hydroxysulfosuccinimide to the highest possible specifications to produce the most specific bioconjugates, to ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of Sulfo-NHS is tested to meet the following minimum specifications:

• Purity—Greater than 95% by quantitative NMR (the highest standard for crosslinker purity);
average lot purity is greater than 99%
• Solubility—Sample dissolves at 2 mg/mL in deionized water to yield a clear, colorless solution
• Identity—IR scan shows only peaks characteristic of N-hydroxysulfosuccinimide

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Pierce™ Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)
NHS (N-hydroxysuccinimide)
EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine-Carboxyl
CleavableNo
DescriptionSulfo-NHS
Molecular Weight (g/mol)217.13
PEGylatedNo
Cell PermeabilityNo
Shipping ConditionAmbient
Product LinePierce™
Labeling MethodChemical Labeling
Crosslinker TypeHeterobifunctional
Reactive MoietyCarbodiimide
SpacerShort (<10 Å)
FormPowder
Quantity5 g
SolubilityWater
FormatStandard, Single-use, Premium-grade
Water SolubleYes
Product TypeCrosslinker
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.

Frequently asked questions (FAQs)

Can NHS and EDC stock solutions be made for long-term storage before use?

No. EDC is very unstable in aqueous environments and must be dissolved immediately before use. NHS and Sulfo-NHS are relatively stable in solution but best results are obtained when they are dissolved immediately before use. Store these compounds desiccated at 4°C.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are there any substances that interfere with NHS reactions?

Yes. Except for the intended targets, reactions must not contain carboxyl or amine compounds. Thus, Tris, glycine, lysine, ethanolamine or other amine- containing buffers must be avoided.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the difference between NHS and Sulfo-NHS?

Sulfo-NHS is the sulfonate sodium salt of NHS; it is water-soluble, but not membrane-permeable. NHS is membrane-permeable and water-soluble. NHS is soluble in organic solvents, as is Sulfo-NHS to a lesser extent. Because NHS is a leaving group in reactions with primary amines, the final conjugation product resulting from NHS and Sulfo-NHS reactions is identical. Sulfo-NHS is chosen for its ability to confer better solubility to the activated compound and/or to control its membrane permeability.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How are NHS and Sulfo-NHS used?

These compounds are used to modify a carboxyl group to form an amine-reactive ester. This is accomplished by mixing NHS with a carboxyl-containing molecule and the carbodiimide EDC (Cat. No. 22980, 22981, 77149, A35391). EDC causes a dehydration reaction between the carboxyl and the NHS hydroxyl group, giving rise to an NHS-ester-activated molecule. The activated molecule can then be reacted spontaneously with a primary amine-containing molecule. Although the carboxyl-molecules can be made to react directly with amines using EDC, the reaction is much more efficient with NHS because a stable intermediate is created. In fact, molecules that are activated as NHS esters can be dried and stored for later reaction to amine-containing targets.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are NHS and Sulfo-NHS used for?

These compounds are used in conjunction with the crosslinker EDC (Cat. No. 22980, 22981, 77149, A35391) to synthesize amine-reactive labeling reagents, crosslinkers and conjugation compounds. Any compound containing a carboxylic acid (-COOH), such as a protein, or biotin or peptide, can be activated with NHS or Sulfo-NHS to form an NHS ester that will spontaneously react to form covalent amide linkages with proteins and other molecules that contain primary amines (-NH2).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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