GelCode™ Blue Stain Reagent
GelCode™ Blue Stain Reagent
GelCode™ Blue Stain Reagent
GelCode™ Blue Stain Reagent
GelCode™ Blue Stain Reagent
Thermo Scientific™

GelCode™ Blue Stain Reagent

GelCode Blue Stain Reagent is a ready-to-use protein stain based on colloidal coomassie dye G-250 that provides nanogram-level detection in polyacrylamide gels and for in-blot detection.
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Catalog NumberDescription
24590GelCode™ Blue Stain Reagent, 500 mL
24592GelCode™ Blue Stain Reagent, 3.5 L
Catalog number 24590
Price (USD)
125.00
Each
Add to cart
Description:
GelCode™ Blue Stain Reagent, 500 mL
Request bulk or custom format
Price (USD)
125.00
Each
Add to cart
GelCode Blue Stain Reagent is a ready-to-use protein stain based on colloidal coomassie dye G-250 that provides nanogram-level detection in polyacrylamide gels and for in-blot detection. This unique reagent stains only protein and allows bands to be viewed directly in the gel during the one-hour gel-staining process.
GelCode Blue Stain Reagent is a ready-to-use protein stain based on colloidal coomassie dye G-250 that provides nanogram-level detection in polyacrylamide gels and for in-blot detection.

Features include:
• No methanol-acetic acid destaining required
• Fast, one-step, one-hour staining, ready-to-use stain reagent; band development visible directly in the staining tray; no background from overnight staining; flexible fixing and washing protocol; no gel shrinkage; stained gel can be dried
• Water wash increases staining sensitivity; optional step provides crystal-clear gel background and makes weakly stained bands more visible

GelCode Blue Stain Reagent stains only protein and allows bands to be viewed directly in the gel during the one-hour gel-staining process. Because the destaining step does not fix the protein, GelCode Blue Stain is compatible with mass spectrometry analysis and N-terminal sequence analysis.

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

GelCode Blue Stain Reagent contains additives that help to slow down the formation of dye-dye and dye-protein aggregates, which form in all coomassie dye-based protein staining reagents. Nevertheless, if left undisturbed, the reagent will form visible dye-dye aggregates that settle in the bottom of the bottle. Fortunately, gently inverting or tipping-and-swirling the bottle several times completely disperses these aggregates. Therefore, it is good practice to mix the stain reagent before pouring or dispensing to ensure that a homogeneous sample of the reagent is used.

Product References:

  1. Mateer, S.C.,et al.(2002).J. Biol. Chem.227,12324-12333.
  2. Aulak, K.S.,et al.(2001).Proc. Natl. Acad. Sci. USA98,12056-12061.
  3. Lim, J.,et al.(2002).J. Biol. Chem.277,20774-20782.
  4. Hilton, J.M.,et al.(2001).J. Biol. Chem.276,16341-16347.
  5. Tani, M.,et al.(2002).J. Biol. Chem.275,3462-3468.
Specifications
DescriptionGelCode™ Blue Stain Reagent, 500 mL
Detection LocationIn-Blot Detection, In-Gel Detection
Detection MethodColorimetric
Label or DyeCoomassie
Product TypeProtein Gel Stain Reagent
Shelf Life6 Months
Product LineGelCode™
Quantity500 mL
Target MoleculeProtein
Unit SizeEach
Contents & Storage
Store at 4°C.

Frequently asked questions (FAQs)

Does GelCode Blue Stain Reagent contain coomassie G-250 or R-250 dye and what is the difference?

GelCode Blue Stain Reagent contains coomassie G-250 dye. Coomassie G (green)-250 is greenish, stains bright blue, is less soluble and used in colloidal form (stain enters a protein better than the acrylamide, so background is minimized). Coomassie R (red)-250 dye is reddish, stains dark blue and is more soluble, usually resulting in high background.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is there a faster staining method using GelCode Blue Stain Reagent?

Yes. The product instructions include a microwave procedure for faster staining with only a minimal loss in sensitivity. Protein bands can be detected approximately 30 minutes after electrophoresis.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do any gel types require fixing before staining with the GelCode Blue Stain Reagent?

Yes. For gels electrophoresed with MOPS or MES running buffers, fix gel with 50% methanol and 7% acetic acid for 15 minutes and extensively wash with water.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can a GelCode Blue Reagent-stained gel also be stained with silver stain?

Yes. The GelCode Blue Reagent-stained gel must be fixed overnight with 30% methanol and 10% acetic acid, followed by washing with water for 1 hour; then the gel is then ready for staining with a silver stain (e.g., Cat. No. 24612).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can GelCode Blue Stain Reagent be used for isoelectric focusing (IEF) gels?

Yes. IEF gels can be stained, but they first must be fixed in 20% TCA for 30 minutes followed by washing with water for 30 minutes; then the gel can be stained as usual.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.