Pierce™ Silver Stain for Mass Spectrometry
Pierce™ Silver Stain for Mass Spectrometry
Thermo Scientific™

Pierce™ Silver Stain for Mass Spectrometry

Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamideRead more
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Catalog number 24600
Price (USD)
314.00
Each
Add to cart
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Price (USD)
314.00
Each
Add to cart
Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis. This kit enables both first-time and experienced users to achieve consistent and reliable staining using high-, low-, and gradient-percentage gels in 1D and 2D formats. The optimized staining method ensures extremely sensitive staining while minimizing covalent crosslinking of protein to the gel matrix, which can inhibit protein recovery. The destaining reagents facilitate complete removal of silver from stained protein bands and maximum protein recovery for subsequent mass spectrometry analysis.

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Features:
• Sensitivity—0.25 ng per spot in 30 minutes after fixing
• MS compatible—reagents and protocol are optimized to provide excellent performance in mass spectrometry following extraction of peptides from stained 1D or 2D gels (SDS-PAGE)
• Flexible staining protocol—fix gel for 15 to 30 minutes or overnight without any effect on staining performance; stain for 1 to 30 minutes (typically 2 to 3 minutes)
• Compatibility—effective silver stain for even difficult-to-stain basic proteins, such as lysozyme (pI 10) and chymotrypsinogen A (pI 9.2), which are detectable at 0.2 ng and 0.5 ng, respectively
• Complete kit—contains all reagents necessary for staining and destaining before in-gel proteolysis and peptide recovery for analysis by mass spectrometry

Silver staining methods generally use either glutaraldehyde or formaldehyde, which cause some covalent crosslinking of protein to each other and the gel matrix. To the extent that this crosslinking occurs, extraction or elution of protein from the gel will be inhibited. Pierce Silver Stain uses formaldehyde in the stain and developer working solutions. However, the procedure accompanying the kit for mass spectrometry is optimized to maximize polypeptide recovery without greatly sacrificing sensitivity.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodColorimetric
Label or DyeSilver
Product TypeSilver Stain
Product LinePierce™
Quantity1 L
Target MoleculeProtein
Unit SizeEach
Contents & Storage
Kit Contents:
• Silver Stain Sensitizer, 2 mL
• Silver Stain Enhancer, 25 mL
• Silver Stain Developer, 500 mL
• Silver Stain, 500 mL
• Silver Destain Reagent A, 4 mL
• Silver Destain Reagent B, 14 mL

Storage: Upon receipt store at ambient temperature

Frequently asked questions (FAQs)

I used the Thermo Scientific Silver Stain for Mass Spectrometry and am getting very high background. Why is this?

Here are possible causes and solutions:

- Stained gel was overdeveloped. Reduce development time.
- Washing step(s) was missed or poor quality water was used. Do not skip or reduce wash steps and use ultrapure water.
- Contaminated equipment was used. Use clean equipment rinsed with ultrapure water.
- Impure chemical was used for gel preparation or precast gel has expired. Use analytical grade chemicals or use precast gels that have not expired.
- Stop solution was not effective in halting development of gel. Prepare new 5% acetic acid and replace it twice in the first minutes of incubation with the gel.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the Thermo Scientific Silver Stain for Mass Spectrometry and my bands are very faint or not visible at all. What should I do?

Here are possible causes and solutions:

- Insufficient development time. Develop gel for more than 5 minutes or add freshly prepared Developer Working Solution.
- Minimal or no protein present in sample. Check protein concentration in the original sample.
- Improper solution preparation or skipped steps. Check solution preparation and follow procedure.
- Excessive water wash before development step. Wash gel three times for 10 minutes each to completely remove the previous solutions and redo the staining procedure starting at step 2. Do not over wash prior to incubation in the developer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How should I process gels stained with Thermo Scientific Silver Stain for Mass Spectrometry, for mass spectrometry analysis?

After using the Thermo Scientific Silver Stain for Mass Spectrometry, proteins can be recovered and processed by several methods for mass spec analysis. For in-gel trypsin digestion, with or without reduction and alkylation, use the Thermo Scientific In-Gel Tryptic Digest Kit (Cat No. 89871). Consider following the recommendations of the core facility that will be performing the mass spec analysis.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the sensitivity of the Thermo Scientific Silver Stain for Mass Spectrometry?

The Thermo Scientific Silver Stain for Mass Spectrometry provides subnanogram sensitivity, detecting proteins at less than 0.25 ng per band in 30 mins after fixing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How should I store the Thermo Scientific Silver Stain for Mass Spectrometry and what is its shelf life?

We recommend storing the Thermo Scientific Silver Stain for Mass Spectrometry at room temperature where it is stable for a year.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.