Imperial Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteinsRead more
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24615
1 L
24617
3 x 1 L
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Catalog number 24615
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184.00
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Quantity:
1 L
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Price (USD)
184.00
Each
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Imperial Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels or nitrocellulose membranes.
Features: • Sensitive—detects less than 3 ng protein per band with the enhanced protocol (3 hours) • Time to stain—ready-to-use reagent detects less than 6 ng protein per band in just 20 minutes • High contrast—intense purple bands are easier to photograph or scan than typical coomassie blue stains • Compatibility—compatible with downstream mass spectrometry analysis and protein sequencing • Additional reagents—water washes only; no acid-fixative or methanol destaining solutions required • Stable—1-year room-temperature stability
The stain is a unique formulation of coomassie brilliant blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercial stains. Staining results in intensely colored protein bands that are easy to photograph and document with gel imagers. This reagent is one of the most sensitive colorimetric stains available, easily detecting 3 to 6 nanograms of protein per band. The easy-to-follow protocol is flexible to meet demanding time and sensitivity requirements.
For Research Use Only. Not for use in diagnostic procedures.
I am seeing visible aggregates in my bottle of Imperial Protein Stain. Is this normal?
Imperial Protein Stain contains additives that help slow formation of dye-dye aggregates, which occur in all Coomassie dye-based protein staining reagents. If left undisturbed, the reagent may form visible aggregates that settle in the bottle; however, gentle mixing completely disperses these aggregates. To ensure that a homogeneous sample of the reagent is used, mix reagent before pouring or dispensing.
After staining my protein gel with Imperial Protein Stain, I am not seeing any band development. What went wrong?
Here are possible causes and solutions:
- No protein was present in sample. Load a known amount of purified protein as a control.
- Insufficient amount of protein in sample. Load more total protein in gel.
- SDS not completely removed from gel. Wash gel more extensively before staining.
Is the Imperial Protein Stain compatible with mass spectrometry analysis?
Yes, here (https://tools.thermofisher.com/content/sfs/brochures/TR0050-Stained-gels-for-MS.pdf) is the procedure for processing gels stained with Imperial Stain, for mass spectrometry analysis.