Imperial™ Protein Stain
Imperial™ Protein Stain
Thermo Scientific™

Imperial™ Protein Stain

Imperial Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteinsRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
246151 L
246173 x 1 L
Catalog number 24615
Price (USD)
184.00
Each
Add to cart
Quantity:
1 L
Request bulk or custom format
Price (USD)
184.00
Each
Add to cart
Imperial Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels or nitrocellulose membranes.

Features:
Sensitive—detects less than 3 ng protein per band with the enhanced protocol (3 hours)
Time to stain—ready-to-use reagent detects less than 6 ng protein per band in just 20 minutes
High contrast—intense purple bands are easier to photograph or scan than typical coomassie blue stains
Compatibility—compatible with downstream mass spectrometry analysis and protein sequencing
Additional reagents—water washes only; no acid-fixative or methanol destaining solutions required
Stable—1-year room-temperature stability

Compare all Coomassie stains ›

The stain is a unique formulation of coomassie brilliant blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercial stains. Staining results in intensely colored protein bands that are easy to photograph and document with gel imagers. This reagent is one of the most sensitive colorimetric stains available, easily detecting 3 to 6 nanograms of protein per band. The easy-to-follow protocol is flexible to meet demanding time and sensitivity requirements.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Blot Detection, In-Gel Detection
Detection MethodColorimetric
Label or DyeCoomassie
Product TypeProtein Stain
Shelf Life1 Year
Product LineImperial™
Quantity1 L
Target MoleculeProtein
Unit SizeEach
Contents & Storage
Upon receipt store product at room temperature.

Frequently asked questions (FAQs)

I am seeing visible aggregates in my bottle of Imperial Protein Stain. Is this normal?

Imperial Protein Stain contains additives that help slow formation of dye-dye aggregates, which occur in all Coomassie dye-based protein staining reagents. If left undisturbed, the reagent may form visible aggregates that settle in the bottle; however, gentle mixing completely disperses these aggregates. To ensure that a homogeneous sample of the reagent is used, mix reagent before pouring or dispensing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

After staining my protein gel with Imperial Protein Stain, I am not seeing any band development. What went wrong?

Here are possible causes and solutions:

- No protein was present in sample. Load a known amount of purified protein as a control.
- Insufficient amount of protein in sample. Load more total protein in gel.
- SDS not completely removed from gel. Wash gel more extensively before staining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I stained my protein gel with Thermo Scientific Imperial Protein Stain and am getting very high background. Do you have any tips?

This is most likely due to SDS interference. We recommend washing the gel extensively before starting the staining procedure.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is the Imperial Protein Stain compatible with mass spectrometry analysis?

Yes, here (https://tools.thermofisher.com/content/sfs/brochures/TR0050-Stained-gels-for-MS.pdf) is the procedure for processing gels stained with Imperial Stain, for mass spectrometry analysis.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the sensitivity of the Imperial Protein Stain?

With the enhanced protocol, the Imperial Protein Stain can detect less than 3 ng protein per band in 3 hours.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.