PageBlue™ Protein Staining Solution
PageBlue™ Protein Staining Solution
Thermo Scientific™

PageBlue™ Protein Staining Solution

PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins inRead more
Have Questions?
Catalog number 24620
Price (USD)
167.00
Each
Add to cart
Request bulk or custom format
Price (USD)
167.00
Each
Add to cart
PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.

Features:
• Easy-to-use—simply soak the gel in the ready-to-use solution and observe stained bands without destaining
• Staining time—25 to 40 minute protocol
• Safe—does not contain methanol or acetic acid
• Dynamic range—linear dynamic range of 5 to 500 ng
• Economical—can be reused up to three times

Compare all Coomassie stains ›

The simple PageBlue Protein Staining Solution protocol is sensitive, efficient, and eliminates the concern of over-staining gels, even after overnight staining. This protein stain delivers a dynamic range of 5 to 500 ng, which is approximately ten times more sensitive than traditional coomassie R-250-based dyes.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Blot Detection, In-Gel Detection
Detection MethodColorimetric
Label or DyeCoomassie
Product TypeProtein Staining Solution
Product LinePageBlue™
Quantity1 L
Target MoleculeProtein
Unit SizeEach
Contents & Storage
Upon receipt store product at room temperature. Product shipped at ambient temperature

Frequently asked questions (FAQs)

After staining my PVDF membrane with PageBlue Protein Staining Solution, I am getting high background on the membrane. Can you offer some tips?

This is most likely due to insufficient destaining time. We recommend destaining the membrane in 30% acetonitrile/20% ethanol solution for an additional 5 mins.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

After staining my PVDF membrane with PageBlue Protein Staining Solution, I am not seeing any band development. What went wrong?

This is most likely due to a problem with the western transfer. Please confirm that the transfer buffer and transfer conditions are correct.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

After staining my protein gel with PageBlue Protein Staining Solution, I am not seeing any band development. What went wrong?

Here are possible causes and solutions:

- No protein was present in sample. Load a known amount of purified protein as a control.
- Insufficient amount of protein in sample. Load more total protein in gel.
- SDS not completely removed from gel. Wash gel more extensively before staining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I stained my protein gel with Thermo Scientific PageBlue Protein Staining Solution and am getting very high background. Do you have any tips?

This is most likely due to SDS interference. We recommend increasing the number of washes and/or wash volumes before staining. Destaining the gel for 5 minutes with 25% isopropanol/10% acetic acid solution or 12% trichloroacetic acid will also help.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do you have any special recommendations for staining small proteins (less than 10 kDa) with the PageBlue Protein Staining Solution?

Small proteins (less than 10 kDa) are susceptible to leaching from the gel during the staining procedure and require fixation with glutaraldehyde before staining the gel with PageBlue Protein Staining Solution. Other common protein fixatives (e.g., acetic acid, isopropanol, ethanol, TCA, etc.) are not suitable for this purpose, as proteins will be washed out of the gel during the staining procedure. Please see procedure for fixation, mentioned on Page 3 of the manual.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all PageBlue™ Protein Staining Solution FAQs