L-Glutamine (200 mM)
L-Glutamine (200 mM)
Gibco™

L-Glutamine (200 mM)

L-Glutamine is an amino acid that is required for cell culture. L-Glutamine participates in the formation of purine and pyrimidineRead more
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Catalog NumberQuantity
25030081100 mL
2503014920 mL
2503016420 x 100 mL
Catalog number 25030081
Price (USD)
38.25
Each
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Quantity:
100 mL
Customize this product
Price (USD)
38.25
Each
Add to cart
L-Glutamine is an amino acid that is required for cell culture. L-Glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. Unlike most other amino acids, L-glutamine is not stable in solution. The rate at which degradation proceeds is a function of time, temperature, and pH. Gibco L-glutamine is a ready-to-use 200 mM stock solution that acts as a cell culture supplement. The optimal concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2–6 mM.

We also offer Gibco GlutaMAX Supplement as a stable alternative to L-glutamine.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture this product at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
Specifications
Chemical Name or MaterialGlutamine
Shelf Life24 Months
pH5 to 6
Recommended StorageStorage conditions: -5°C to -20°C. Protect from light.
Shipping conditions: Dry ice
Shelf life: 24 months from date of manufacture
Concentration or Composition (by Analyte or Components)100X
Quantity100 mL
Physical FormLiquid
Product LineGibco™
Shipping ConditionDry Ice
Green FeaturesSustainable packaging
Unit SizeEach

Frequently asked questions (FAQs)

Why did my 200 mM L-Glutamine precipitate out of solution when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution have “Product of Brazil” on the label?

L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution is made in the USA from a raw material glutamine powder manufactured in Brazil. The country of manufacture of a component that has been subjected to manufacturing or processing operations in more than one country is the last country in which the component has been “substantially transformed”. A “substantial transformation” occurs in a country when a product emerges from a manufacturing process with a name, character, or use different from that of the original material(s) subjected to that process.

Diluting, purifying, sorting, testing, packing, repacking, or affixing labels to a product in a country does not constitute a “substantial transformation” in that country, hence the label must bear the country of the original raw material origin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My order of L-Glutamine (Cat. No. 25030081) arrived frozen. Is it stable?

If L-Glutamine is completely or partially frozen, it is still stable. It becomes unstable when stored for extended periods of time completely thawed, especially above 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is your recommended method for thawing L-Glutamine before adding to cell culture media?

We recommend thawing at 2-8 degrees C and then warming at 37 degrees C until the material goes into solution. Mix it as it warms up to spend minimal time at elevated temperatures before you aliquot it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why did my 200 mM L-glutamine precipitate when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37 degrees C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.