PMPI (p-maleimidophenyl isocyanate)

Thermo Scientific Pierce PMPI is a maleimide-and-isocyanate crosslinker for attaching compounds to sulfhydryl groups (cysteines) after conjugating the linker to hydroxyl groups on a molecule by urethane (carbamate) bonds.

Features of PMPI:

• Reactive groups: maleimide and isocyanate
• Reactive towards: sulfhydryl and hydroxyl groups
• Short (8.7A), sulfhydryl-to-hydroxyl crosslinker with an aromatic spacer arm (noncleavable)
• Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
• Isocyanate reacts with non-aqueous hydroxyl groups to form carbamate linkage
• Excellent tool for conjugating hydroxyl-containing compounds such as steroids and vitamins
• Targets and reaction solutions must not contain primary amines