Leibovitz's L-15 Medium, GlutaMAX™ Supplement
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Gibco™

Leibovitz's L-15 Medium, GlutaMAX™ Supplement

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue.Read more
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Catalog NumberQuantity
31415029500 mL
Catalog number 31415029
Price (USD)
60.75
Each
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Quantity:
500 mL
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Price (USD)
60.75
Each
Add to cart

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration.


This L-15 is modified as follows:
WithWithout
• Galactose• Glucose
• Phenol Red• HEPES
• GlutaMAX™• Sodium Bicarbonate
• Sodium Pyruvate 


The complete formulation is available.

Gibco™ L-15 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

L-15 contains no proteins, lipids, or growth factors. Therefore, L-15 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Culture EnvironmentNon-CO2
ClassificationAnimal Origin-free
Concentration1 X
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeLeibovitz's L-15 Medium
Shelf Life12 Months
SterilitySterile-filtered
With AdditivesGlutaMAX, Phenol Red, Sodium Pyruvate, Galactose
Without AdditivesNo HEPES, No Sodium Bicarbonate, No Glucose
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality300 - 340 mOsm/kg
pH7.4 to 7.9
Product LineGibco™, GlutaMAX™
Quantity500 mL
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

Frequently asked questions (FAQs)

What is the osmolality of Leibovitz's L-15 Medium?

We do provide osmolality information on the certificate of analysis. All lots of Leibovitz's L-15 Medium will meet the osmolality specification of 300-340 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Leibovitz's L-15 Medium, GlutaMAX™ Supplement FAQs