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Pierce™ ECL Western Blotting Substrate
Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) thatRead more
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| Catalog Number | Quantity | Unit Size |
|---|---|---|
| 32106 | 500 mL | 500 mL |
| 32109 | 50 mL | Each |
| 32209X4 | 4 x 250 mL | Each |
| 32209 | 250 mL | Each |
| 32106X4 | 4 x 500 mL | Each |
| 32106TS also known as 32106 | Each |
Catalog number 32106
Price (USD)
285.00
500 mL
Quantity:
500 mL
Unit Size:
500 mL
Price (USD)
285.00
500 mL
Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
Pierce ECL Plus Western Blotting Substrate characteristics include:
• Sensitivity: low picogram
• Stability: 1-hr working solution stability, 1-year kit stability at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 1 to 2 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 μg/mL)
• Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 μg/mL)
Compare and learn more about our western blot chemiluminescent substrates ›
View all HRP substrates available ›
Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.
Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.
Pierce ECL Plus Western Blotting Substrate characteristics include:
• Sensitivity: low picogram
• Stability: 1-hr working solution stability, 1-year kit stability at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 1 to 2 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 μg/mL)
• Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 μg/mL)
Compare and learn more about our western blot chemiluminescent substrates ›
View all HRP substrates available ›
Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.
Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPierce ECL Western Blotting Substrate
Product TypeSubstrate
Detection MethodChemiluminescence
FormLiquid
For Use With (Application)Western Blot
Membrane CompatibilityNitrocellulose, PVDF
Product LinePierce™
Quantity500 mL
ConjugateHRP
SubstrateHorseradish Peroxidase
Recommended Antibody Concentrations2°: 1:1-15K (0.07-1μg/mL), 1°: 1:1K (0.2- 10μg/mL)
SensitivityLow Picogram
Signal Duration1 to 2 hr.
Sufficient For5000 sq. cm
Kit ContentsLuminol/Enhancer, 250mL, Stable Peroxide Buffer, 250mL
Unit Size500 mL
Contents & Storage
Upon receipt store at 2-8°C. Product is shipped at ambient temperature.