Dynabeads™ M-270 Epoxy, for OEM and industrial use only
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Thermo Scientific™

Dynabeads™ M-270 Epoxy, for OEM and industrial use only

Dynabeads™ M-270 Epoxy are monosized 2.8-μm superparamagnetic polystyrene beads coated with a cross-linked layer of hydrophilic polyether. Epoxy functional groupsRead more
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Catalog NumberQuantity
34003D1 g
34004D10 g
Catalog number 34003D
Price (USD)
-
Quantity:
1 g

Dynabeads™ M-270 Epoxy are monosized 2.8-μm superparamagnetic polystyrene beads coated with a cross-linked layer of hydrophilic polyether. Epoxy functional groups allow for covalent coupling of antibodies or other proteins, peptides, lectins, haptens, or functional enzymes onto a non-charged bead surface through amine or thiol groups with no further activation of the surface. No blocking protein is needed during the preparation, and standard assay diluents may be used. The Dynabeads™-ligand complex can be used for immunoassays or any other application requiring an active biomolecule conjugated to a magnetic solid support.

Dynabeads™ magnetic beads are widely used as a solid-phase for presenting antibodies or antigens in automated clinical immunoassay systems, where the combination of specific antigens or antibodies and the superparamagnetic properties of the beads provide rapid reaction kinetics both in the coating process, separation, and during washing of the analyte.

The product is provided in a freeze-dried format and meets reputable Dynal high standards with respect to reproducibility and automation ability, and drives reliability for your assays.

Benefits:
• Efficient and reproducible immobilization of antibodies (or other ligands)
• Easy and reproducible handling in manufacturing
• Rapid separation and washing
• Reproducible behavior in automation
• Easy coupling without need for protein blocking
• Freeze-dried beads
• High activity of bound antibody and antigen
• Easy assay optimization
• Well-suited for binding of small antigens such as synthetic or recombinant peptides
• Generally low background and high signal-to-noise ratio

Applications
Ideally suited for magnetic bead-based immunoassays requiring a low background signal. Also suited for any application involving immunoaffinity binding to antibody or antigen immobilized on a magnetic surface.

Note:
This particular product format is for large volume customers, available on an OEM basis. This product is also available in smaller volumes (14301D and 14302D).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
MaterialPolystyrene
Product TypeEpoxy Bead
Product LineDynabeads™
Quantity1 g
Surface FunctionalityEpoxy, Hydrophilic
Diameter (Metric)2.8 μm
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).

Frequently asked questions (FAQs)

What reactive groups do you offer for your surface-activated Dynabeads magnetic beads?

We offer tosyl, epoxy, carboxylic acid and amine activated Dynabeads magnetic beads. Please see the link (https://tools.thermofisher.com/content/sfs/brochures/Surface_Activated_Dynabeads.PDF) for a comparison of the beads.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the density of Dynabeads magnetic beads?

The density of Dynabeads magnetic beads is a challenging property to determine. The reason is that Dynabeads magnetic beads have a 17-37% magnetic iron oxide content in order to have a reasonable magnetic separation time, and the density of the iron oxide is about 4.9 g/cm3. Dynabeads magnetic beads are composite materials, being a mix of polymers and iron oxide, and there are very few polymers that have a density below 1.

The sedimentation rate depends on the bead diameter squared, so the sedimentation of a 1 µm bead is much slower than that of 2.8 µm. The effect of diameter on sedimentation rate is to some extent counteracted by the fact that smaller beads need to have a higher content of iron oxide for magnetic separation applications. Typically, our M-280 Dynabeads (diameter 2.8 µm) have a density of 1.4 g DS/cm3 (DS = dry substance), our M-270 Dynabeads (diameter 2.8 µm) and M-450 Dynabeads (diameter 4.5 µm) have a density of 1.6 g DS/cm3, and our MyOne Dynabeads (diameter 1 µm) have a density of 1.8 g DS/cm3.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center as well as our Dynabeads Cell Isolation and Expansion Support Center and Protein Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Pulldown Support Center.

I am using surface-activated Dynabeads magnetic beads. When do I need to use a linker secondary antibody?

A linker antibody provides proper orientation of the target-specific primary antibody. Optimal orientation of the primary antibody is more important for reacting with larger organelles than for small organelles or membrane fractions. Different linkers can be used, but we recommend using an Fc-binding antibody, such as a monoclonal or polyclonal anti-mouse IgG. The linker antibody must be affinity purified and not contain stabilizers such as sugars or proteins that may bind to the Dynabeads magnetic beads. The specific primary antibody, if polyclonal, must be affinity purified in order to provide a high density of the specific antibody on the Dynabeads magentic beads surface.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.