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SuperSignal™ West Femto Maximum Sensitivity Substrate
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein levelRead more
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| Catalog Number | Quantity |
|---|---|
| 34096 | 200 mL |
| 34094 | 20 mL |
| 34095X4 | 4 x 100 mL |
| 34095 | 100 mL |
| 34096X4 | 4 x 200 mL |
Catalog number 34096
Price (USD)
714.00
Each
Quantity:
200 mL
Price (USD)
714.00
Each
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme.
SuperSignal West Femto Maximum Sensitivity Substrate characteristics include:
• Sensitivity: low-femtogram (mid-zeptomole)
• Stability: 8-hr working solution stability, 1-year kit stability at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to 8 hours
• Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
• Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)
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SuperSignal West Femto Maximum Sensitivity Substrate is designed for low femtogram-level detection by western blot analysis. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeSubstrate
Detection MethodChemiluminescence
FormLiquid
For Use With (Application)Western Blot
Membrane CompatibilityNitrocellulose, PVDF
Product LineSuperSignal™
Quantity200 mL
ConjugateHRP
SubstrateHorseradish Peroxidase
Recommended Antibody Concentrations2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
SensitivityLow femtogram-level
Signal Duration6 to 8 hr.
Sufficient For25 mini-gel blots, 2000 sq. cm of membrane
Kit ContentsStable Peroxide Buffer (100 mL), Luminol/Enhancer (100 mL)
Unit SizeEach
Contents & Storage
Sufficient For: 25 mini-gel blots; 2000 cm2 of membrane
• Luminol/Enhancer, 100 mL
• Stable Peroxide Buffer, 100 mL
Store reagents at 4°C.
• Luminol/Enhancer, 100 mL
• Stable Peroxide Buffer, 100 mL
Store reagents at 4°C.