Pierce™ Fast Western Kit, SuperSignal™ West Pico, Mouse
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Thermo Scientific™

Pierce™ Fast Western Kit, SuperSignal™ West Pico, Mouse

Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate contains optimized reagents and protocol for picogram-level detection andRead more
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Catalog NumberDescription
35065Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate, Mouse Trial Size
35060Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate, Mouse
Catalog number 35065
Price (USD)
159.00
Each
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Description:
Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate, Mouse Trial Size
Request bulk or custom format
Price (USD)
159.00
Each
Add to cart
Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate contains optimized reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Features of this kit:

Fast—picogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—cost as little as $14 per blot; no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kits contain all components needed to block, probe and develop a blot with your mouse primary antibody

These Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Pico Chemiluminescent Substrate.The kits contain all the reagents necessary to complete a Western blot being probed with either a mouse or rabbit primary antibody. Like other Western blotting methods, the included SuperSignal West Pico Substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed.

The Pierce Fast Western Blot Kits are reagent-based system that provide optimized reagents for blocking, antibody dilution and detection of Western blots. The Fast Western Blot Kit protocol is quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other popular rapid Western blotting systems.

Related Products
Pierce™ Fast Western Kit, SuperSignal™ West Pico, Rabbit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPierce Fast Western Blot Kit, SuperSignal West Pico Substrate, Mouse Trial Size
Product TypeWestern Blot Kit
Detection MethodChemiluminescence
FormLiquid
For Use With (Application)Western Blot
Membrane CompatibilityNitrocellulose, PVDF
Product LinePierce™, SuperSignal™
Quantity200 mL
ConjugateHRP
SubstrateHorseradish Peroxidase
ReactivityMouse
SensitivityPicogram-level
Sufficient For5 Mini Blots
Unit SizeEach
Contents & Storage
Sufficient For: 5 mini blots probed with mouse primary antibodies
• Fast Western Antibody Diluent, 100 mL
• Fast Western 10X Wash Buffer, 50 mL
• Fast Western Mouse Optimized HRP Reagent, Pico, 5 mL
• SuperSignal West Pico Luminol/Enhancer Solution, 25 mL
• SuperSignal West Stable Peroxide Solution, 25 mL

Store kit at 4°C.

Frequently asked questions (FAQs)

How can I increase the sensitivity of my Western blot?

Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.