Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Mouse
Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Mouse
Thermo Scientific™

Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Mouse

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate provides reagents and protocol for reliable and rapid,Read more
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Catalog number 35070
Price (USD)
526.00
Each
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Price (USD)
526.00
Each
Add to cart
The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate provides reagents and protocol for reliable and rapid, 1-hour chemiluminescent Western blotting at femtogram levels of sensitivity.

Features of this kit:

Fast—femtogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kits contain all components needed to block, probe and develop a blot with your mouse primary antibody

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classical Western blotting with SuperSignal West Dura Chemiluminescent Substrate. Like other Western blotting methods, the included SuperSignal West Dura Substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse primary antibody.

Related Products
Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Rabbit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPierce Fast Western Blot Kit, SuperSignal West Dura Substrate, Mouse
Product TypeWestern Blot Kit
Detection MethodChemiluminescence
FormLiquid
For Use With (Application)Western Blot
Membrane CompatibilityNitrocellulose, PVDF
Product LinePierce™, SuperSignal™
Quantity400 mL
ConjugateHRP
SubstrateHorseradish Peroxidase
ReactivityMouse
SensitivityFemtogram-level
Sufficient For10 western blots (800 sq. cm of membrane)
Unit SizeEach
Contents & Storage
Sufficient For: 10 mini blots probed with mouse primary antibodies
• Fast Western Antibody Diluent, 200 mL
• Fast Western 10X Wash Buffer, 100 mL
• Fast Western Mouse Optimized HRP Reagent, Dura, 10 mL
• SuperSignal West Dura Luminol/Enhancer Solution, 50 mL
• SuperSignal West Stable Peroxide Solution, 50 mL

Store kit at 4°C.

Frequently asked questions (FAQs)

How can I increase the sensitivity of my Western blot?

Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.