McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement
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Gibco™

McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cellRead more
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Catalog NumberQuantity
36600021500 mL
Catalog number 36600021
Price (USD)
81.75
Each
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Quantity:
500 mL
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Price (USD)
81.75
Each
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McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.


This McCoy's 5A is modified as follows:
WithWithout
• High Glucose• Sodium Pyruvate
• GlutaMAX™• HEPES
• Bacto-peptone 
• Phenol Red 


The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator.

Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications
Culture EnvironmentCO2
Cell TypeMammalian Cells
ClassificationAnimal Origin-free
Concentration1 X
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeMcCoy's 5A Medium
Shelf Life12 Months
SterilitySterile-filtered
With AdditivesHigh Glucose, GlutaMAX, Phenol Red, Bacto-peptone, Sodium Bicarbonate
Without AdditivesNo HEPES, No Sodium Pyruvate
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality280 - 320 mOsm/kg
pH6.9 to 7.1
Product LineGibco™, GlutaMAX™
Quantity500 mL
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

Frequently asked questions (FAQs)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement FAQs