Blocker™ Casein
Blocker™ Casein
Thermo Scientific™

Blocker™ Casein

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods.
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Catalog NumberDescription
37532Blocker Casein in TBS, 1 L
37582Blocker Casein in PBS, 100 mL
37528Blocker Casein in PBS, 1 L
37583Blocker Casein in TBS, 100 mL
37528X3Blocker Casein in PBS, 3 x 1 L
Catalog number 37532
Price (USD)
189.00
Each
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Description:
Blocker Casein in TBS, 1 L
Request bulk or custom format
Price (USD)
189.00
Each
Add to cart
Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods. Blocker Casein is 1% (w/v) casein, which corresponds to the optimal concentration for most applications.
Features of Blocker Casein include:
• Purified casein—single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions
• Easy to use—1% casein solution is ready to use; can be diluted further as needed
• Safe—stable, thimerosal-free formulation

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionBlocker Casein in TBS, 1 L
Product TypeBlocker Casein
Quantity1 L
FormLiquid
For Use With (Application)Western Blot, ELISA
Product LineBlocker™
Concentration1X
BufferTBS
Blocking AgentSingle purified protein
Unit SizeEach
Contents & Storage
Store at 4°C.

Frequently asked questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the main ingredient in Blocker Casein (Cat. No. 37583, 37532)?

Blocker Casein (Cat. No. 37583, 37532) is a ready-to-use, 1% (w/v) solution of purified casein in Tris-buffer saline (TBS). It is a stable, thimerosal-free formulation and is already at the optimal concentration for most applications. However, it can be diluted further if needed.

Find additional tips, troubleshooting help, and resources within our Protein Biology Support Centers .