Thermo Scientific SuperBlock Blocking Buffer, blotting is an optimized PBS solution containing a protein that provides incredibly fast and effective blocking for western blot analysis.
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Catalog Number
Buffer
37537
TBS
37517
PBS
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Catalog number 37537
Price (USD)
230.00
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Buffer:
TBS
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Price (USD)
230.00
Each
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Thermo Scientific SuperBlock Blocking Buffer, blotting is an optimized PBS solution containing a protein that provides incredibly fast and effective blocking for western blot analysis. Blocking micro-plates, membranes, or tissues with SuperBlock buffer yields a high signal-to-noise ratio in most detection systems.
Features of SuperBlock Blocking Buffer, blotting include: • Fast—block membranes in 5 to 10 minutes • Flexible—guaranteed to be biotin-free for use with streptavidin system • Low background—the non-serum protein solution yields a high signal-to-noise ratio • Stable—store buffer at 4°C for one year; store blocked plates dry for up to 12 months
Blocking micro-plates, membranes, or tissues with SuperBlock buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.
For Research Use Only. Not for use in diagnostic procedures.
Upon receipt store at 4°C. Product is shipped at ambient temperature.
Frequently asked questions (FAQs)
How can I reduce background bands in my Western blot?
Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.