StartingBlock™ Blocking Buffer
StartingBlock™ Blocking Buffer
Thermo Scientific™

StartingBlock™ Blocking Buffer

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays.
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Catalog NumberDescription
37579StartingBlock (TBS) Blocking Buffer, 100 mL
37578StartingBlock (PBS) Blocking Buffer, 100 mL
37538StartingBlock (PBS) Blocking Buffer, 1 L
37538X3StartingBlock (PBS) Blocking Buffer, 3 x 1 L
37542StartingBlock (TBS) Blocking Buffer, 1 L
37539StartingBlock T20 (PBS) Blocking Buffer, 1 L
37543StartingBlock T20 (TBS) Blocking Buffer, 1 L
Catalog number 37579
Price (USD)
56.75
Each
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Description:
StartingBlock (TBS) Blocking Buffer, 100 mL
Request bulk or custom format
Price (USD)
56.75
Each
Add to cart
StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock Blocking Buffer is available in PBS and TBS, with and without Tween 20.
Features of StartingBlock Blocking Buffer include:
Strip and re-probe without re-blocking—blots stay blocked even after stripping with Restore Stripping Buffer (Cat. No. 21059)
Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum- and biotin-free)
Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes; almost instantaneous for polystyrene microplate wells

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionStartingBlock (TBS) Blocking Buffer, 100 mL
Product TypeBlocking Buffer
Quantity100 mL
FormLiquid
For Use With (Application)Western Blot, ELISA, Immunohistochemistry (IHC)
Product LineStartingBlock™
FormulationProprietary protein formulation in Tris-buffered saline at pH 7.5
Concentration1X
BufferTBS
Blocking AgentSingle purified protein
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.

Frequently asked questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.