SuperBlock™ Blocking Buffer
SuperBlock™ Blocking Buffer
Thermo Scientific™

SuperBlock™ Blocking Buffer

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.
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Catalog NumberDescription
37581SuperBlock (TBS) Blocking Buffer, 100 mL
37580SuperBlock (PBS) Blocking Buffer, 100 mL
37515SuperBlock (PBS) Blocking Buffer, 1 L
37518SuperBlock (PBS) Blocking Buffer, 5 L
37535SuperBlock (TBS) Blocking Buffer, 1 L
37545SuperBlock (TBS) Blocking Buffer, dry blend, 5 packs
37516SuperBlock T20 (PBS) Blocking Buffer, 1 L
37536SuperBlock T20 (TBS) Blocking Buffer, 1 L
Catalog number 37581
Price (USD)
57.25
Each
Add to cart
Description:
SuperBlock (TBS) Blocking Buffer, 100 mL
Request bulk or custom format
Price (USD)
57.25
Each
Add to cart
SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis. Blocking micro-plates, membranes, or tissues with a SuperBlock buffer yields a high signal-to-noise ratio in most detection systems.
Features of SuperBlock Blocking Buffer include:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes, or tissues with SuperBlock buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionSuperBlock (TBS) Blocking Buffer, 100 mL
Product TypeBlocking Buffer
Quantity100 mL
FormLiquid
For Use With (Application)ELISA, Western Blot
Product LineSuperBlock™
Concentration1X
BufferTBS
Blocking AgentSingle purified protein
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C. Product is shipped at ambient temperature.

Frequently asked questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.