Pierce™ Clear Milk Blocking Buffer (10X)
Pierce™ Clear Milk Blocking Buffer (10X)
Thermo Scientific™

Pierce™ Clear Milk Blocking Buffer (10X)

Pierce Clear Milk Blocking Buffer (10X) is a long-shelf life, high-performance replacement for homemade non-fat milk blocking buffers in westernRead more
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Catalog number 37587
Price (USD)
201.00
Each
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Price (USD)
201.00
Each
Add to cart
Pierce Clear Milk Blocking Buffer (10X) is a long-shelf life, high-performance replacement for homemade non-fat milk blocking buffers in western blotting applications.

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Our Clear Milk Blocking Buffer (10X) is a pre-formulated milk solution, clarified and stabilized for blocking excess nonspecific binding sites, reducing background in western blotting applications and diluting antibodies when used with nitrocellulose and PVDF membranes. It provides lower background, enhanced sensitivity, extended shelf life, and reproducible results compared to homemade buffers based on dissolved non-fat dry milk. Not recommended for avidin-based techniques because it contains some endogenous biotin.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeBlocking Buffer
Quantity100 mL
FormLiquid
For Use With (Application)Western Blot
Concentration10X
BufferBorate
Blocking AgentClarified milk proteins
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.

Frequently asked questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.