SYBR™ Green Universal Master Mix
Try PowerTrack SYBR Green Master Mix - our newest formulation simplifies your workflow by minimizing primer optimization and reducing pipetting errors with an optional sample tracking dye.
SYBR™ Green Universal Master Mix
Applied Biosystems™

SYBR™ Green Universal Master Mix

Notification of updated SYBR Green master mix formulation: PowerTrack SYBR Green Master Mix is our newest formulation designed to greatlyRead more
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Catalog NumberQuantity
43349731 x 50 mL
43444631 x 1 mL
43091551 x 5 mL
43643442 x 5 mL
43643465 x 5 mL
431270410 x 5 mL
Catalog number 4334973
Price (USD)
4,895.00
Each
Add to cart
Quantity:
1 x 50 mL
Price (USD)
4,895.00
Each
Add to cart

Notification of updated SYBR Green master mix formulation: PowerTrack SYBR Green Master Mix is our newest formulation designed to greatly simplify your work and deliver high sensitivity and specificity. It comes with an optional yellow dye that can be added to your DNA sample for pipetting QC. Yellow sample added to blue master mix yields green qPCR reaction. Visually confirm that your DNA sample was added to master mix. PowerTrack SYBR Green Master Mix is compatible with a wide range of primer melting temperatures (55°C to 65°C) and concentrations to minimize primer optimization work.

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SYBR Green PCR Master Mix
Everything you need for SYBR Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems SYBR Green PCR Master Mix combines SYBR Green I dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

  • Premixed components stored at 2–8°C significantly reduce assay setup time
  • SYBR Green I dye detects double-stranded DNA, so specific probes are not required
  • AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
  • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
  • Proprietary buffer enhancements ensure performance and reliability

Maximum flexibility and convenience
Applied Biosystems SYBR Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan probes are required. SYBR Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR–related fluorescence fluctuations. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodSYBR
FormatTube
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
PolymeraseAmpliTaq Gold DNA Polymerase
Reaction SpeedStandard
For Use With (Equipment)7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Label or DyeSYBR Green
Passive Reference DyeROX (Pre-mixed)
Product LineSYBR™
Product TypeReal Time PCR SYBR Master Mix
Quantity1 x 50 mL
Shipping ConditionWet Ice
Sufficient For2000 Reactions of 50 μL
For Use With (Application)Gene Expression
Concentration2X
No. of Reactions2000 Reactions
Sample TypeDNA (Genomic), cDNA
Volume1 x 50 mL
Unit SizeEach
Contents & Storage
The 1 x 50 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 2000 reactions based on a 50 μl reaction volume.

Store at -20°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Frequently asked questions (FAQs)

When using SYBR Green dye chemistry on an Applied Biosystems 7900HT Fast, a 7500 or 7500 Fast, or a 7300 Real-Time PCR System, is there an option to set the quencher to reflect the fact that there is no TaqMan probe being used in the reaction?

Yes. On the Detector Manager for the System, set the Quencher to “Non Fluorescent”.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

Is the use of AmpErase UNG necessary for performing reverse transcription reactions?

No. We do not recommend that you use AmpErase UNG when performing reverse transcription. When using a dNTP mix with dUTP in a RT reaction, uracil will get incorporated into the cDNA generated from your RNA template. AmpErase UNG (uracil N-glycosylase), through an enzymatic reaction, will cleave single or double stranded DNA dUTP containing sequences.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

Can Power SYBR Green PCR Master Mix (Cat. No. 4368577, 4367659, 4368706, 4368702, 4368708, 4367660) be substituted directly for the SYBR Green PCR Master Mix (Cat. No. 4309155, 4364344, 4364346, 4312704, 4334973)?

The Power SYBR Green PCR Master Mix is a new and improved formulation of the regular SYBR Green PCR Master Mix. You may need to verify your assays in order to start using the Power SYBR Green PCR Master Mix. For more information on verifying your reaction with the Power SYBR Green PCR Master Mix, please refer to the Power SYBR Green PCR Master Mix protocol.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What is the difference in sensitivity between TaqMan chemistry vs. SYBR Green reagent chemistry?

Sensitivity can actually be equivalent when using TaqMan chemistry and SYBR Green reagent chemistry. It might seem that a TaqMan assay with fluorescent signal generated by a sequence-specific probe would always be more sensitive than a SYBR Green reagent assay, but a poorly designed TaqMan assay could theoretically be less specific than a well-designed SYBR Green reagent assay. However, the potential for detection of primer dimers and non-specific products using SYBR Green chemistry is more likely to result in loss of sensitivity when attempting to quantitate lower copy numbers.

For more information on Real-Time PCR chemistries, please refer to the following Application Notes, which you can find on our website through Technical Resources, or by entering the titles in the main Search field: “Real-Time PCR Vs. Traditional PCR”, “Essentials of Real Time PCR”, and “Selection of Reagents for Real-Time PCR”.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What are the key differences between a TaqMan MGB probe and a TaqMan TAMRA dye-labeled probe?

The TaqMan MGB probes contain the following features:
1) A fluorescent reporter at the 5' end
2) A nonfluorescent quencher at the 3' end. Because the quencher does not fluoresce, the real-time instruments can measure the reporter dye contributions more precisely.
3) A minor groove binder at the 3' end. The minor groove binder increases the melting temperature (Tm) of probes, allowing the use of shorter probes.

In general, the TaqMan MGB probes exhibit great differences in Tm values between matched and mismatched probes, which provides more accurate allelic discrimination and makes for a more sensitive real-time assay. Mismatches between a probe and allele, or target, reduce the efficiency of probe hybridization in a measurable way, which is especially important in SNP Genotyping assays. Furthermore, AmpliTaq Gold DNA polymerase is more likely to displace the mismatched probe rather than cleave it to release reporter dye. More information about TaqMan MGB probes can be found in the User Bulletin entitled "Primer Express Version 1.5 and TaqMan MGB Probes for Allelic Discrimination." You can find a copy on our website by entering this title in the main search field.

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