POP-7™ Polymer for 3730/3730xl DNA Analyzers
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Applied Biosystems™

POP-7™ Polymer for 3730/3730xl DNA Analyzers

POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencingRead more
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Catalog NumberQuantity
433561130 x 28 mL
43639291 x 28 mL
43356155 x 28 mL
436393510 x 28 mL
Catalog number 4335611
Price (USD)
17,190.00
Each
Add to cart
Quantity:
30 x 28 mL
Price (USD)
17,190.00
Each
Add to cart
POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencing and fragment analysis. Not suitable for use with ABI PRISM ™, 310 and 3100 series genetic analyzers. It causes syringe failure and premature array degradation. Sufficient for 8,000 - 12,000 samples.

POP-7™ Polymers for the 3730⁄3730xl Genetic Analyzers is:
• Pre-formulated, which not only saves time but also saves reproducibility by ensuring consistent formulation
• Formulated for sequencing and fragment analysis applications
• Designed to allow capillaries to be used multiple times

Reproducible Results
POP™ polymers dynamically coat the capillary wall to control electro-osmotic flow. Their definite quality and uniform consistency eliminate guesswork and help to ensure reproducibility. POP polymers are specifically formulated to separate DNA fragments of a known size range at a desired resolution and run time.

Used With
3730/3730xl Genetic Analyzer Capillary Array, 50 cm, 3730 Running Buffer, 10X, BigDye™ Terminator v1.1 Cycle Sequencing Kit, BigDye™ Terminator v3.1 Cycle Sequencing Kit, BigDye™ Terminator v3.1 Cycle Sequencing Kit, BigDye™ Terminator v3.1 Cycle Sequencing Kit, Hi-Di™ Formamide.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accuracy98.5% up to 900 bases (for 50 cm capillary), 98.5% up to 700 bases (for 36 cm capillary)
For Use With (Equipment)3730 DNA Analyzer
For Use With (Application)Standard Sequencing, Fragment Analysis (Denaturing)
On Instrument Life UsageStability on instrument 7 days, up to 12,000 samples/28 mL
Product LinePOP-7™
Product TypePolymer
Quantity30 x 28 mL
Test Time1 hr for 36 cm capillary (std. seq.), 2 hrs for 50 cm capillary (long read seq.)
Unit SizeEach
Contents & Storage
Refrigerate at 2°C to 8°C.

Frequently asked questions (FAQs)

How many samples can I run with one 28 ml bottle of POP-7 polymer on my Applied Biosystems 3730 Series DNA Analyzer?

You can run 8,000-12,000 samples. These numbers may be lower if there is increased polymer consumption due to additional wizards being performed, such as the Bubble Remove and Water Wash wizards.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

How long can POP-7 polymer stay on the Applied Biosystems 3730 Series DNA Analyzer?

POP-7 polymer can maintain its performance for seven days on the Applied Biosystems 3730 Series DNA Analyzer at standard lab conditions. After seven days, perform all required maintenance (including the Water Wash Wizard) and replace with fresh polymer. Do not "top off" the polymer bottle (add fresh polymer to old polymer), which can adversely affect data quality and consumable lifespan.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

What polymer is supported on the Applied Biosystems 3730 Series DNA Analyzer?

Applied Biosystems supports the use of POP-7 polymer on the Applied Biosystems 3730 Series DNA Analyzer. If the instrument is running Applied Biosystems 3730 Data Collection Software v.3.1 or later, then you can also use POP-6 polymer. We do not recommend mixing and matching of various instrument polymers as each is manufactured in different processes, tested as an entire system, and guaranteed to perform to a minimum specification. Substitution of reagents may compromise the integrity of the system and cannot be supported by AB service or applications support specialists. Using unsupported polymers may adversely affect data quality, consumable lifespan, and may void existing warranties.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

What is the white residue around the array knob on my Applied Biosystems 3730/3730xl DNA Analyzer?

When placing the array on the instrument, the last step in the process is to loosen the array knob and the instrument forces any air bubbles around the array tip through the threading and the middle of the array knob. Some polymer gets pushed through as well and when it dries up, it appears as a white, flaky residue. Use a damp, lint-free cloth to remove it. If more residue appears after the cleanup, it is possible that the array knob is not tightened enough or the array ferrule may require replacement.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

There is a large bubble at the tip of the piston on my Applied Biosystems 3730/3730xl DNA Analyzer. How can I remove it?

It is not unusual to see a large bubble at the tip of the piston while performing the water wash wizard on the system. When the polymer is put back on to the system, the difference in viscosity is usually enough to dislodge the bubble and push it out. In extreme cases, it may be necessary to repeat the wash and go back and forth between the water and polymer to force it out.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

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