BigDye™ Terminator v3.1 Cycle Sequencing Kit
BigDye™ Terminator v3.1 Cycle Sequencing Kit
Applied Biosystems™

BigDye™ Terminator v3.1 Cycle Sequencing Kit

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishingRead more
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Catalog NumberQuantity
433745424 Reactions
4337455100 Reactions
43374561000 Reactions
43374575000 Reactions
433745825,000 Reactions
Catalog number 4337454
Price (USD)
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Quantity:
24 Reactions

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

  • Improve the quality of your results for a wide range of sequencing applications
  • Optimized for long read lengths
  • Better dye mobility characteristics
  • Improved performance reading through GT-rich regions
  • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
  • Enhance your productivity and reduce costs

Get more uniform peak heights and improved accuracy

With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Format12-well Strips, 8-well Strip, 96- or 384-well Plate
For Use With (Equipment)SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
For Use With (Application)AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing
Product LineBigDye™ Terminator
Product TypeSequencing Buffer
Quantity24 Reactions
TechniqueFluorescent Dye Terminator Sequencing
Template CompatibilityBAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), PCR Amplicons
Unit SizeEach
Contents & Storage
• 200 μL tube of BigDye™ Terminator v3.1 Ready Reaction Mix
• 1 tube M13 (-21) Primer
• 1 tube pREF-BDT™ Control DNA
• 1 mL tube of 5X Sequencing Buffer (may be stored at 4°C)

Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.

Frequently asked questions (FAQs)

What information should I include when submitting data to Genetic Analysis Technical Support?

When contacting Genetic Analysis Support (FAS, FSE, Technical Support), please have (if possible):

Instrument model and serial numbers
Version of the software are you using
Application and/or kit information
Kit lot number
Number of runs on the capillary or array
Polymer type and lot number
Buffer lot number
Hi-Di Formamide lot number or loading solution information
Sample data that can be sent to the support person
Details on:
  -Reaction setup (i.e., how much DNA was used, primer, etc.)
  -How the reactions were cleaned up (alcohol precipitation, columns, etc)
  -What template DNA was used (i.e. plasmid, PCR product)
  -How the template DNA was prepared?

All of this information will help the support person quickly and accurately assess the problem and provide you with recommendations for resolution.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

I have a long homopolymer stretch in my sample that I cannot get through. Do you have any tips for getting through it?

In order to get through homopolymeric regions, you can either use anchored sequencing primers or try using the dRhodamine Terminator Cycle Sequencing Kit since it uses ddTTP instead of ddUTP and has been shown to be less prone to producing stutters, specifically with poly-T regions.

For more information on sequencing through homopolymer stretches, please refer to the DNA Sequencing by Capillary Electrophoresis: Applied Biosystems Chemistry Guide: Second Edition (Cat. No. 4305080, Rev. C). The guide can be found by searching the Thermo Fisher Scientific website with the catalog number 4305080.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

I am trying to sequence GC-rich DNA without success. Do you have any tips for getting through this?

When trying to sequence through GC-rich regions, the following tips may improve your success:

• Set the reaction up at 1X or 0.5X strength. Heavily diluting the BigDye Terminator Ready Reaction mix can reduce the success of the sequecing reaction through long GC stretches.

• Perform a hot start at 98–99°C for 5 minutes.

• Use 5% (w/v) DMSO or freshly made betaine in the reaction.

• Use the dGTP BigDye Terminator Cycle Sequencing Kit. G peaks will appear compressed due to the presence of ddGTP, but sequencing through long GC stretches using the dGTP kit is typically more successful than with the standard BigDye Terminator Cycle Sequencing Kits (containing ddITP). For more information on sequencing GC-rich DNA, please refer to the DNA Sequencing by Capillary Electrophoresis: Applied Biosystems Chemistry Guide: Second Edition (Cat. No. 4305080, Rev. C). The guide can be found by searching the Thermo Fisher Scientific website with the catalog number 4305080.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.