Silencer™ Select GAPDH Positive Control siRNA
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Invitrogen™

Silencer™ Select GAPDH Positive Control siRNA

Ambion™ Silencer™ SelectGAPDH Positive Control siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment.Read more
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Catalog NumberQuantity
43908495 nmol
439085040 nmol
Catalog number 4390849
Price (USD)
264.00
Each
Add to cart
Quantity:
5 nmol
Price (USD)
264.00
Each
Add to cart
Ambion™ Silencer™ SelectGAPDH Positive Control siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment. 5 nmol is provided.

• Premium-quality siRNA, highly purified and ready to use
• Functionally tested in several common cell lines
• Include Silencer™ Select modifications for enhanced specificity
• For use in human, mouse, and rat cells

What are Silencer™ Select siRNAs?
Silencer™ Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Silencer™ Select GAPDH Positive Control siRNA:
The Silencer™ SelectGAPDH positive control is extensively validated and an ideal “test” siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. Because it targets GAPDH mRNA (a common internal control), many assays are available to measure its effects, including real-time RT-PCR as well as the Ambion™ KDalert™ GAPDH Assay Kit.

Quality Control:
Silencer™ Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly.

Accessory Product: siPORT™ NeoFX™ Transfection Agent (SKU #AM4510 and #AM4511)
siPORT™ NeoFX™ Transfection Agent is a versatile, lipid-based transfection agent for the efficient transfection of siRNA into cultured cells. The protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications. Silencer™ Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer™ Select siRNAs. If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion™ Silencer™ siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Control TypePositive Control
RNAi TypesiRNA
For Use With (Application)RNAi, Transfection
Label or DyeUnconjugated
Product LineSilencer™, Silencer™ Select, Ambion™
Product TypesiRNA
PurityHPLC
Quantity5 nmol
Shipping ConditionRoom Temperature
TargetGAPDH
Unit SizeEach
Contents & Storage
The siRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C.

Frequently asked questions (FAQs)

What are the benefits of using a vector to deliver RNAi?

Vector technologies allow you to:

Achieve transient or stable target knockdown
Perform RNAi in any cell type, even hard-to-transfect, primary, and non-dividing cells
Regulate gene inhibition with inducible siRNA expression
Select for a pure population of cells stably expressing an siRNA sequence
Control gene expression in vivo with tissue-specific promoters

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Why are my cells dying after transfection?

We would suggest running a transfection reagent control only to determine if your cells are sensitive to the transfection reagent. Additionally, you can try using different cell densities and siRNA concentrations to diminish any toxic effects from the transfection itself.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I transfected my siRNA and the mRNA levels are down, but the protein is not. Why is that?

In some cases, knockdown of a protein can be affected by other variables such as protein turnover rate, even though the RNA is knocked down. Additionally, a longer time course may be needed to see an effect on protein compared to mRNA.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I am not getting my target knockdown. What could be the cause of this?

Please see the following possibilities and suggestions:

- How many siRNA did you test? Is there any knockdown? If there is no knockdown (<10%) in any of the siRNA, then the assay is likely the problem. Try using a different qRT-PCR assay to assess knockdown.
- What was the positioning of the qRT-PCR assay target site relative to the cut site for the siRNA? If greater than 3,000 bases away, the problem could be alternative splice transcripts.
- What are the Cts for the experiment? They should be below 35 in a 40-cycle qRT-PCR experiment.
- Did you confirm the siRNA got into the cell? We recommend using a validated positive control siRNA to check the transfection efficiency.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I am not getting any knockdown with my siRNA. What do you suggest I try?

Please see the following possibilities and suggestions:

- Were the mRNA levels checked? The most reliable method is real-time PCR. In some cases, knockdown of a protein can be affected by other variables, such as protein turnover rate, even though the RNA is knocked down.
- How is the RNA being isolated? Has the quality of the isolated RNA been checked? Ensure that the RNA has not been degraded.
- Was a positive control used? This can help to determine whether the reagents are working and whether the siRNA was delivered correctly to the cell. Run your experiment in parallel with the positive control siRNA.
- Was a transfection control used? What is the percentage of transfected cells?
- Was a time course used? Generally, gene silencing can be assessed as early as 24 hours posttransfection. However, the duration and level of knockdown are dependent on cell type and concentration of siRNA.
- Was optimization of transfection conditions performed? You can try using different cell densities and siRNA concentrations.
- Which concentration of siRNA did you use? We recommend testing multiple concentrations between 5 nM and 100 nM.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

View all Silencer™ Select GAPDH Positive Control siRNA FAQs