AmpliTaq™ 360 DNA Polymerase
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AmpliTaq™ 360 DNA Polymerase
Applied Biosystems™

AmpliTaq™ 360 DNA Polymerase

Green features
The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.
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Catalog NumberQuantity
4398818250 Units
43988281000 Units
439889725 x 1000 Units
43988955000 Units
Catalog number 4398818
Price (USD)
193.00
Each
Add to cart
Quantity:
250 Units
Request bulk or custom format
Price (USD)
193.00
Each
Add to cart
The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. Compared to the original AmpliTaq DNA Polymerase, AmpliTaq 360 DNA Polymerase is purified by an additional proprietary separation process that reduces contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and, when combined with the proprietary AmpliTaq 360 Buffer Kit, promotes the amplification of a variety of templates, including those from bacterial and human genomes.

Features

  • Optimized for the broadest range of targets, from everyday to challenging
  • Unmatched sensitivity and yield
  • Market-leading GC enhancer for robust amplification of GC-rich sequences
  • Achieves the highest quality sequencing data

Notes

  • DreamTaq DNA Polymerase is recommended for superior PCR performance.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatStand-alone enzyme
GC-Rich PCR PerformanceMedium
PolymeraseAmpliTaq 360 DNA Polymerase
Reaction SpeedStandard
Exonuclease Activity5' - 3'
Green FeaturesSustainable packaging
Product TypeDNA Polymerase
Quantity250 Units
Shipping ConditionRoom Temp or Wet Ice
For Use With (Application)Standard PCR
Concentration5 U/μL
Fidelity (vs. Taq)1X
Hot StartNo
No. of Reactions200 Reactions
Overhang3'-A
Reaction FormatSeparate Components
Size (Final Product)5 kb or less
Starting MaterialDNA
Unit SizeEach
Contents & Storage
• AmpliTaq 360 DNA Polymerase (5 U/μL), 50 μL
• 10X AmpliTaq 360 PCR Buffer, 1.5 mL
• 25 mM MgCl2, 1.5 mL
• 360 GC Enhancer, 1.5 mL

Store at -15°C to -25°C.
Product is guaranteed up to 12 months as indicated by the expiration date.

Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all AmpliTaq™ 360 DNA Polymerase FAQs