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Applied Biosystems™
MeltDoctor™ HRM Reagent Kit
Applied Biosystems™ MeltDoctor™ HRM Reagent Kit provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolutionRead more
Catalog number 4425557
Price (USD)
846.00
Each
Price (USD)
846.00
Each
Applied Biosystems™ MeltDoctor™ HRM Reagent Kit provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.
This kit includes:
• AmpliTaq Gold™ 360 DNA Polymerase which, when combined with AmpliTaq Gold™ 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold™ 360 DNA Polymerase delivers 360° coverage for a full range of targets.
• GeneAmp™ dNTP Blend
• MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes
This kit includes:
• AmpliTaq Gold™ 360 DNA Polymerase which, when combined with AmpliTaq Gold™ 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold™ 360 DNA Polymerase delivers 360° coverage for a full range of targets.
• GeneAmp™ dNTP Blend
• MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodHRM
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
PolymeraseAmpliTaq Gold 360 DNA Polymerase
TechniqueHRM (High Resolution Melting) Analysis, Mutation Scanning
For Use With (Equipment)7500 Fast System, 7900HT Fast System
Genotyping TargetSNPs (Unknown or Numerous), Epigenetic Targets
Label or DyeHigh Resolution Melting (HRM), SYTO 9
Product LineMeltDoctor™
Product TypeHrm Reagent Kit
Quantity1 kit
For Use With (Application)Real Time PCR (qPCR)
Sample TypeDNA (Genomic), cDNA
Unit SizeEach
Contents & Storage
This kit includes: AmpliTaq Gold™ 360 DNA Polymerase (2 x 50 μL, each at 5 U/μL), AmpliTaq Gold™ 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, and MeltDoctor™ HRM Dye (20x). The kit provides sufficient materials for 250 20-μL reactions when using 2 U AmpliTaq Gold 360 DNA Polymerase per reaction.
Store at -15° to -25°
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Store at -15° to -25°
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).