TaqMan™ Non-coding RNA Assay
TaqMan™ Non-coding RNA Assay
Applied Biosystems™

TaqMan™ Non-coding RNA Assay

Green features
Applied Biosystems TaqMan Noncoding RNA assays are used for quantitative real-time PCR analysis of RNA expression and consist of aRead more
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Catalog NumberQuantity
4426961S (360 reactions), made to order
4426962M (750 reactions), made to order
4426963L (2900 reactions), made to order
Catalog number 4426961
Price (USD)
373.00
Each
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Quantity:
S (360 reactions), made to order
Applied Biosystems TaqMan Noncoding RNA assays are used for quantitative real-time PCR analysis of RNA expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end.

Features include:
• Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
• Accurate—identify small fold-changes with high accuracy of quantitation
• Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology

Approximate ship time
Made to order: 4–6 days in North America, 6–10 days in Europe

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents. All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays qPCR Guarantee.*

Recommended master mix: TaqMan Fast Advanced Master Mix

*Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration60 X
Detection MethodPrimer-probe Detection
FormatTube
Green FeaturesSustainable packaging
No. of Reactions360 reactions
PCR MethodqPCR
Product LineTaqMan™
Product TypeNon-Coding RNA Assay
QuantityS (360 reactions), made to order
Reaction SpeedFast
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

Frequently asked questions (FAQs)

Did you take into account sequence homology when you developed the TaqMan Gene Expression Assays product?

During the TaqMan Gene Expression Assays product (including TaqMan Gene Expression Assays and Custom Plus TaqMan RNA Assays) design process, we take the following steps to avoid regions of ambiguity: 1. We BLAST the primer and probe designs against transcript databases to ensure specificity to ensure the chosen assay detects only transcript(s) from the gene of interest. 2. We BLAST primer and probe designs against genome databases in order to avoid assays that detect pseudo-genes or genomic DNA.

What region of my gene of interest does the TaqMan Gene Expression Assay product cover?

You can view TaqMan Gene Expression Assay product coverage on the Assay details page. To do this, search for your gene of interest, for example "Cox".  On your results page, select the "Assay ID" for a particular TaqMan Gene Expression Assay; for example: Hs00187909_m1.  The resulting page is the assay details page and will list regions covered by the TaqMan Gene Expression Assay.

When preparing the TaqMan or Custom TaqMan Gene Expression Assays, what is the recommended reaction volume?

When preparing the TaqMan or Custom TaqMan Gene Expression Assays, the recommended and supported volume is 20-50 µL for a 96-well standard plate set-up; 10-20 µL for a 96-well fast plate set-up and 5-10 µL for a 384-well set-up. For more information, please consult the TaqMan Gene Expression Assays and Custom TaqMan Gene Expression Assays protocols.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

How much cDNA is recommended per TaqMan or Custom TaqMan Gene Expression reaction?

For optimal performance of the TaqMan and Custom TaqMan Gene Expression Assays, use 1 to 100 ng of cDNA per 50 µL total reaction volume. For more information, please consult the TaqMan Gene Expression Assay protocol and Custom TaqMan Gene Expression Assay protocol.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

Can I use SYBR Green I dye in conjunction with the TaqMan Gene Expression Assays or Custom TaqMan Gene Expression Assays?

No, SYBR Green dye I is not compatible with the TaqMan Gene Expression Assays or Custom TaqMan Gene Expression Assays. TaqMan Gene Expression Assays or Custom TaqMan Gene Expression Assays contain TaqMan MGB probes combined with primers at non-limiting concentrations. The probes already have detection dyes conjugated to them, and SYBR Green is not needed. For more information, please consult the protocol of TaqMan Gene Expression Assays and Custom TaqMan Gene Expression Assays.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

View all TaqMan™ Non-coding RNA Assay FAQs