ViiA™ 7 Real-Time PCR System with TaqMan™ Array Block
Notice: This product is discontinued. See QuantStudio 7 Flex Real-time PCR System »
ViiA™ 7 Real-Time PCR System with TaqMan™ Array Block
Applied Biosystems™

ViiA™ 7 Real-Time PCR System with TaqMan™ Array Block

Green features
The ViiA™ 7 Real-Time PCR System combines all of the qPCR features you want in a single high performance instrument,Read more
Have Questions?
Catalog number 4453537
Price (USD)
-
The ViiA™ 7 Real-Time PCR System combines all of the qPCR features you want in a single high performance instrument, so that you can optimize your research productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, the ViiA™ 7 System offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or fast-cycling 384- or 96-well plates and TaqMan™ Array Micro Fluidic Cards and Reagents makes high-productivity real-time PCR accessible to any size lab.

• Proven Applied Biosystems reliability and accuracy.
• Using with TaqMan™ Array Micro Fluidic Cards provides an integrated workflow and faster results.
• Easy to use – intuitive software, responsive touch-screen, automation, effortless block exchange without the need for any tools.

Productivity
Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 System enhances your lab’s productivity.
• Quick block changes: Front access makes it easy to change thermal cycling block formats without having to move attached peripherals such as robots or computers.
• Easy touch-screen interface: Instrument touch screen provides one-touch protocols for fast and easy assay setup for a broad range of applications.
• Automation compatibility: Integrated with Applied Biosystems™ Twister™ II Robot, the ViiA™ 7 System allows you to maximize productivity for automated environments.
• Peace of mind: Data recovery capability for 100+ runs.

Integration
Fully Compatible with TaqMan™ Array Micro Fluidic Cards, Applied Biosystems™ TaqMan™ Assays are the gold standard in qPCR analysis, providing high sensitivity, high specificity, and a broad dynamic range.
• TaqMan™ Array-ready: Hundreds of customizable and predefined TaqMan™ Array Micro Fluidic Cards are available, containing preloaded TaqMan™ Gene Expression or MicroRNA Assays in an easy-to-use format; you can analyze 1–8 samples in parallel using your choice of 12–384 TaqMan™ Assays
• 384-well throughput without robotics: With TaqMan™ Array Micro Fluidic Cards, there’s no need for liquid-handling robotics or complex pipetting to load; simply add your sample and master mix and run on the ViiA™ 7 System
• Compatible with the full range of TaqMan™ Assays: The ViiA™ 7 System is optimized to enable clear, clean data for all TaqMan™ Assays, including MicroRNA Assays, Protein Assays, Long Non-coding RNA Assays, and Pri-miRNA Assays

Performance
The ViiA™ 7 System features the new OptiFlex™ System, which provides enhanced fluorescence detection enabling accurate and sensitive data analysis.
• Better accuracy: High-resolution detection system for more accurate reads with TaqMan™ Array Micro Fluidic Cards, 384-well plates, and 96-well plates.
• Maximum multiplexing: Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities.
• Flexible data collection: Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage.
• Precise quantification: Detect as small as 1.5–fold changes in target quantities in singleplex reactions.

ViiA™ 7 Software
ViiA™ 7 software drives value in a number of ways, offering:
• Intuitive interface and innovative design
• Convenient walk-away operation
• Improved data analysis
• Fully compatible with high-throughput environments

Expandable Software Architecture
Optional add-ons to upgrade features to existing software
• 21 CFR Part 11 compliance: The optional SAE module assists with Security, Auditing and Electronic signature of records for full data traceability.
• High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Block FormatInterchangeable
Format384-well Microfluidic Card
SensitivityDown to 1 Copy
Temperature Range (Metric)4 to 100°C
Reaction Volume Range1 μl
Dynamic RangeUp to 9 Logs of Linear Dynamic Range
High-throughput CompatibilityHigh-throughput Compatible, Multiplexing
For Use With (Equipment)ViiA™ 7 System
Thermal Uniformity±0.5°C
Reaction SpeedStandard
Detection MethodPrimer-Probe Detection
Run TimeAbout 35 min. (varies depending on Reagents and Other Factors)
Optics6 Emission Filters (500 to 720 nm), 6 Excitation Filters (450 to 670 nm), OptiFlex™ System (Enhanced Fluorescence Detection)
Dimensions53.3 cm (21 in.) (W) x 63.5 cm (25 in.) (D) x 64.5 cm (25.4 in.) (H)
Capacity1 Array Card
Voltage100 to 240 V
Electrical Requirements60 Hz
Product LineViiA™
Display TypeTouchscreen
Green FeaturesEnergy Efficient, Fewer Resources used and Less Waste
Other Format Options96-well Fast Block Upgrade Option, 384-well Block Upgrade Option, 96-well Standard Block Upgrade Option
ProgramsRemote Monitoring of up to 10 Instruments
ResolutionDetects as little as 1.5-fold changes in target quantities (in singleplex reactions)
Plate HandlingAutomation includes Plate-loading Arm with Barcode, Optional Automation Accessory Robot
Quantity1 instrument
Weight60.7 kg
Unit SizeEach
Contents & Storage
This instrument comes with a TaqMan™ Array Block.

Frequently asked questions (FAQs)

How do I activate or renew my software license for the ViiA 7 Real-Time PCR System?

In the ViiA 7 Real-Time PCR System, please follow the instructions found in the Software Licensing Quick Reference Guide at https://fnoclient.gss.tf/quick-start-guide to activate or renew a license. If you have a license key associated with a computer that is no longer in use, please follow the instructions to "Transfer a license to a different user or computer" in the above guide.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What can I do when I get the error - "Array index out of range: 1" in the ViiA 7 Real-Time PCR System?

This error can occur when the run was started from a corrupt template file or if the file name is very long or has illegal characters. Delete any templates that were created in v1.1 or earlier. Upgrade your software to the most current version. Recreate your templates in v1.2 or later. Only use templates created in v1.2 or later for any future runs. If the file name is very long, try using the “Save As” feature to resave the file with a shorter name. If this does not fix the problem, please send your file to techsupport@thermofisher.com for further assistance.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why am I not able to see/export Quantity or Ct values in the ViiA 7 Real-Time PCR System?

After exporting data into .xls format, the Quantity or Ct values may not be present. From your file, Select ‘Export‘ and click on ‘Load Export Set' and then ‘OK' and ‘OK'. Then click on ‘Start Export'. The Quantity or Ct values should now be present in the exported file.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does the error "Data collection incomplete and cannot be handled by analysis…" mean?

This error is caused by a corrupt template or when the ‘Open Run Method' button was selected to start the run. To avoid this, do not use corrupt templates or run files from previous software versions. If you have v.1.1 or earlier, please upgrade to the most current version. If you are using templates created from v.1.1 or earlier, please delete and recreate them in v1.2 or above. New run files created from v1.2 can use the ‘Open Run Method' button.
If you have a file that gives you this error, please send your data file (*.eds) to techsupport@thermofisher.com for assistance.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

On the ViiA 7 instrument, what does the error “Calibration file already exists” mean?

The calibration files are stored on the ViiA 7 instrument itself; therefore, when you need to perform a new calibration, you will have to delete the old calibration files. From the instrument console, go to the ViiA 7 instrument touchscreen and select ‘Collect Results'. You should then see a list of files stored on the instrument. Manually select a calibration file such as “Fast-96-cal”, and press the ‘Delete' button to remove the old calibration file. Occasionally, users cannot delete the file off the instrument and may encounter this error: “Some experiments did not collect results therefore cannot be deleted.”. In these situations, launch the software, go to ‘maintenance', manage files, and download the calibration file. The status of the experiment should be displayed as ‘complete'. Next, delete the old calibration file and rerun the calibration.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.