POROS™ XQ Strong Anion Exchange Resin
POROS™ XQ Strong Anion Exchange Resin
Thermo Scientific™

POROS™ XQ Strong Anion Exchange Resin

Thermo Scientific™ POROS™ XQ resin is a next-generation, high-capacity, high-resolution, salt-tolerant, strong anion exchange resin that that enables >140 mg/mL dynamic binding capacity in the presence of up to 6.6 mS/cm NaCl, while delivering exceptional separation performance.
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Catalog NumberQuantity
4467820250 mL
44678181 L
44678175 L
446781610 L
Catalog number 4467820
Price (USD)
1,490.00
Each
Add to cart
Quantity:
250 mL
Price (USD)
1,490.00
Each
Add to cart

POROS XQ resin is a next-generation, high-capacity, high-resolution, salt-tolerant, strong anion exchange resin that that enables >140 mg/mL dynamic binding capacity in the presence of up to 6.6 mS/cm NaCl, while delivering exceptional separation performance. A major improvement to the performance of traditional anion exchangers, POROS XQ resin sets the standard for strong anion exchange chromatography in both bind & elute or flow-through modes of operation.

Features of POROS XQ resin include:

  • High dynamic binding capacity leading to reduced column size, smaller footprint, decreased water and buffer usage, and reduced cycling
  • High resolution supporting improved product yield and purity, ultimately improving the cost of goods for the molecule being produced
  • Robust salt tolerance allowing enhanced process design flexibility and streamlining of process flow
  • Low operating back pressure and linear pressure versus flow responses driving flexible scalability
  • Rigid polymeric bead with covalent surface chemistry for easier handling and packing, and superior physical and chemical stability enabling a more robust downstream purification process

Dynamic Binding Capacity

POROS XQ resin has a high binding capacity of more than 140 mg/mL at 5% breakthrough, over a broad range of process conditions, with superior binding capacity across a broad range of pH and salt conditions. Higher dynamic binding capacity was achieved by optimizing the base bead pore structure and surface chemistry.

High Resolution

Using an optimized 50 μm particle size, POROS XQ resin maintains the superior resolving capacity of the POROS resins, overcoming the challenging impurity levels associated with high titer processes. Resolution remains consistent across a wide range of operating flow rates.

Robust Salt Tolerance

POROS XQ resin has high and consistent protein capacity across a broad range of salt concentrations up to 140 mg/mL at 50 mM NaCl (6.6 mS/cm conductivity). The ability to load columns under higher conductivity reduces or helps eliminate the dilution of column feed streams or the removal of unit operations, such as tangential flow filtration.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Recommended StorageRoom Temperature
Particle Size50 μm
PackagingBottle
Quantity250 mL
Stationary PhaseStrong anion exchange
Product LinePOROS™
Unit SizeEach

Frequently asked questions (FAQs)

Are POROS analytical columns compatible with HPLC systems from Waters or Agilent?

All of the pre-packed POROS columns, regardless of column size or resin, have the same 10-32 female fittings on the column. Columns are compatible with Waters and Agilent HPLC systems. You will need 1/16 inch male fittings for the HPLC to attach the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For any POROS analytical column, is the column compatible with an AKTA or Avant purification system?

All of the pre-packed columns, regardless column size or resin, have the same 10-32 female fittings on the column. They are all compatible with the AKTA instrument platform, however due to the column volume of the 2.1 mmD x 30 mmL columns (0.1 mL), these will not perform well in AKTA or Avant purification systems due to the relative size of the hold- up volume in these systems. The smallest column (PEEK or SS) that could feasibly be used in these systems is the 4.6mmD x 50mmL (0.8 mL) format.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are the pre-packed POROS CaptureSelect or any POROS analytical columns compatible with an UPLC or is the use of these columns limited to HPLC?

UPLC is typically described as chromatography with sub-2 µm particles, which drives higher efficiency/better separation independent of operating flow rate. As particle size decreases, backpressure increases so the UPLC systems tend to have higher pressure ratings compared to HPLC systems. The flow rates on UPLC systems would be sufficient for POROS columns. Pump capabilities are typically 0.1-1.0 mL/min, so not as fast as a HPLC, but sufficient. Hold-up volumes on UPLC systems are lower than HPLCs, so this can help with sensitivity and efficiency. So from a pressure and flow standpoint there should be no challenge to operate a POROS CaptureSelect column on a UPLC system.

That being said, care should be taken running POROS columns on UPLC systems. Higher pressure safety interlock MUST be set at the rated column pressure, typically 180 bar. HPLC column hardware is not rated to operate at the static pressures possible and typical on UPLC systems. A clog or the like on a UPLC system could generate unsafe pressures on any HPLC-rated column before the default UPLC safety pressure interlock is tripped.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the physical or mechanical stability of POROS resins?

The physical stability of POROS resins is high. Customers have agitated, with overhead mixing and or bottom gassing for long periods of time (5-10 hours) and have performed hundreds of diaphragm pump passes during stall packs. Keep in mind that agitation with any device that creates grinding and sheer, such as with a magnetic stir bar, is not recommended for any type of resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should I do if a POROS column dries out or air is pumped onto the column?

POROS resins rehydrate quite well. Here is the procedure that we recommend:

1. Flow 5% ethanol over the column at ?350 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
2. If baseline noise is still present, flow 20% ethanol over the column at 300 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
3. If you have established column qualification tests and specifications, perform the tests after rewetting.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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