POROS™ GoPure™ 50 D Pre-packed Column, 0.5 x 5 cm, 1 mL
POROS™ GoPure™ 50 D Pre-packed Column, 0.5 x 5 cm, 1 mL
Thermo Scientific™

POROS™ GoPure™ 50 D Pre-packed Column, 0.5 x 5 cm, 1 mL

Thermo Scientific™ POROS™ GoPure™ 0.5 cm pre-packed columns are intended for the screening of POROS ion exchange resins or for small scale purification.
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Catalog number 4481319
Price (USD)
144.00
Each
Add to cart
Price (USD)
144.00
Each
Add to cart

POROS GoPure 0.5 cm pre-packed columns are intended for the screening of POROS ion exchange resins or for small scale purification.

These pre-packed columns are ready to connect and have the following features:

  • Dimensions of 0.5 x 5 cm, with a nominal bed volume of 1 mL, providing a uniform screening platform
  • Direct connection to standard chromatography systems
  • Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The resin in this POROS GoPure D 50 μm Column has a dimethylaminopropyl functional group for strong anion exchange chromatography.

  • Convenient—ready-to-run columns simplify and speed POROS resin evaluations
  • Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
  • Simplified scalability—the consistency and range of the POROS GoPure family of pre-packed columns simplifies process scale-up and process modeling

POROS GoPure 0.5 cm pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended ≥3 min residence time (0.3 mL/min) on this 5 cm length column equates to 400 cm/hr on a 20 cm bed height. Detailed specifications may be found in the POROS GoPure 0.5 cm Operating Instructions below.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography™ resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, POROS GoPure Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 mL to 6.3 liters.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity1 column
Particle Size50 μm
For Use With (Equipment)FPLC
Bed Volume1 mL
Product TypeGoPure Pre-packed Column
Diameter (Metric) Inner0.5 cm
Max. Pressure30 bar
Sample TypeBiopharmaceutical Purification Sample, Cell Culture Supernatants, Serum
Stationary PhaseDimethylaminopropyl
Product LineGoPure™
Column TypeAnion Exchange
High-throughput CompatibilityAutomated Protocols
Unit SizeEach
Contents & Storage
Shipped in 20% ethanol at 2°C to 30°C.

Frequently asked questions (FAQs)

Are POROS analytical columns compatible with HPLC systems from Waters or Agilent?

All of the pre-packed POROS columns, regardless of column size or resin, have the same 10-32 female fittings on the column. Columns are compatible with Waters and Agilent HPLC systems. You will need 1/16 inch male fittings for the HPLC to attach the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For any POROS analytical column, is the column compatible with an AKTA or Avant purification system?

All of the pre-packed columns, regardless column size or resin, have the same 10-32 female fittings on the column. They are all compatible with the AKTA instrument platform, however due to the column volume of the 2.1 mmD x 30 mmL columns (0.1 mL), these will not perform well in AKTA or Avant purification systems due to the relative size of the hold- up volume in these systems. The smallest column (PEEK or SS) that could feasibly be used in these systems is the 4.6mmD x 50mmL (0.8 mL) format.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are the pre-packed POROS CaptureSelect or any POROS analytical columns compatible with an UPLC or is the use of these columns limited to HPLC?

UPLC is typically described as chromatography with sub-2 µm particles, which drives higher efficiency/better separation independent of operating flow rate. As particle size decreases, backpressure increases so the UPLC systems tend to have higher pressure ratings compared to HPLC systems. The flow rates on UPLC systems would be sufficient for POROS columns. Pump capabilities are typically 0.1-1.0 mL/min, so not as fast as a HPLC, but sufficient. Hold-up volumes on UPLC systems are lower than HPLCs, so this can help with sensitivity and efficiency. So from a pressure and flow standpoint there should be no challenge to operate a POROS CaptureSelect column on a UPLC system.

That being said, care should be taken running POROS columns on UPLC systems. Higher pressure safety interlock MUST be set at the rated column pressure, typically 180 bar. HPLC column hardware is not rated to operate at the static pressures possible and typical on UPLC systems. A clog or the like on a UPLC system could generate unsafe pressures on any HPLC-rated column before the default UPLC safety pressure interlock is tripped.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the physical or mechanical stability of POROS resins?

The physical stability of POROS resins is high. Customers have agitated, with overhead mixing and or bottom gassing for long periods of time (5-10 hours) and have performed hundreds of diaphragm pump passes during stall packs. Keep in mind that agitation with any device that creates grinding and sheer, such as with a magnetic stir bar, is not recommended for any type of resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should I do if a POROS column dries out or air is pumped onto the column?

POROS resins rehydrate quite well. Here is the procedure that we recommend:

1. Flow 5% ethanol over the column at ?350 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
2. If baseline noise is still present, flow 20% ethanol over the column at 300 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
3. If you have established column qualification tests and specifications, perform the tests after rewetting.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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