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SMART Digest™ ImmunoAffinity (IA) Protein A Kits
Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well for your biomarker studies.
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| Catalog Number | Description |
|---|---|
| 60111-103 | SMART Digest IA Kit, Protein A magnetic with SOLAμ SPE and collection plate |
| 60111-101 | SMART Digest IA Kit, Protein A non-magnetic |
| 60111-102 | SMART Digest IA Kit, Protein A non-magnetic with SOLAμ SPE and collection plate |
| 60111-104 | SMART Digest IA Kit, Protein A magnetic |
Catalog number 60111-103
Price (USD)
1,788.37
Each
Description:
SMART Digest IA Kit, Protein A magnetic with SOLAμ SPE and collection plate
Price (USD)
1,788.37
Each
Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Protein A Kits. These kits were designed to remove the challenges often associated with the process of biomarker quantitation by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight. The SMART Digest IA Protein A Kits target purification of immunoglobulins due to the strong affinity for binding to the Fc portion of the molecule.
Solving the challenges of low-level quantitation in complex matrices
The need to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging.
As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time consuming, multistep, and prone to irreproducibility.
Immunocapture and Protein Digestion in a Single WellThese IA kits deliver a process that is:
- Fast
- Simple
- Highly reproducible
- Sensitive
In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.
Specifications
DescriptionSMART Digest IA Kit, Protein A magnetic with SOLAμ SPE and collection plate
CaptureProtein A
CleavageC-terminal side of lysine and arginine amino acids
EnzymeTrypsin
FormatSMART Digest Resin, Magnetic
For Use With (Application)Peptide Quantitation
Product LineSMART Digest
TypeImmunocapture + Protein Digestion combined on a single bead
IncludesMagnetic SMART Digest Resin, Collection Plate, SOLAμ SPE Plate
Unit SizeEach