SMART Digest™ Trypsin Kits
SMART Digest™ Trypsin Kits
Actual product may vary
Thermo Scientific™

SMART Digest™ Trypsin Kits

Obtain fast, simple, and highly reproducible trypsin protein digestion for peptide characterization and quantitation in biopharmaceutical protein research.

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Catalog NumberDescriptionFormatIncludes
60113-101SMART Digest Trypsin Kit, SolubleSMART Digest Soluble TrypsinSMART Digest Soluble Trypsin, SMART Digest Buffer, Collection Plate
60109-101SMART Digest Trypsin Kit, with Collection plateSMART Digest Resin Pre-filled into PCR TubesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate
60109-101-LPHSmart digest kit with collection plate, low pHSMART Digest Low pH Resin Pre-filled into PCR TubesSMART Digest Low pH Resin Pre-filled into 96 PCR Tubes, Collection Plate
60109-103SMART Digest Trypsin Kit, with SOLAμ/collection plateSMART Digest Resin Pre-filled into PCR TubesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate, SOLAμ SPE Plate
60109-102SMART Digest Trypsin Kit, with Filter/Collection plateSMART Digest Resin Pre-filled into PCR TubesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate, Filter Plate
60109-101-BSMART Digest Trypsin Kit, Bulk Resin optionSMART Digest Resin, Bulk Non-magneticBulk Non-magnetic SMART Digest Resin, SMART Digest Buffer
60109-101-MBSMART Digest Trypsin Kit, Magnetic Bulk Resin optionSMART Digest Resin, Bulk MagneticBulk Magnetic SMART Digest Resin, SMART Digest Buffer
60109-102-BSMART Digest Trypsin Kit, Bulk Resin option with Filter/Collection plateSMART Digest Resin, Bulk Non-magneticBulk Non-magnetic SMART Digest Resin, SMART Digest Buffer, Collection Plate, Filter Plate
60109-102-MBSMART Digest Trypsin Kit, Magnetic Bulk Resin option with Filter/Collection plateSMART Digest Resin, Bulk MagneticBulk Magnetic SMART Digest Resin, SMART Digest Buffer, Collection Plate, Filter Plate
60109-103-BSMART Digest Trypsin Kit, Bulk Resin option with SOLAμ/collection plateSMART Digest Resin, Bulk Non-magneticBulk Non-magnetic SMART Digest Resin, SMART Digest Buffer, Collection Plate, SOLAμ SPE Plate
60109-103-MBSMART Digest Trypsin Kit Magnetic Bulk Resin option with SOLAμ/collection plateSMART Digest Resin, Bulk MagneticBulk Magnetic SMART Digest Resin, SMART Digest Buffer, Collection Plate, SOLAμ SPE Plate
Catalog number 60113-101
Price (USD)
818.83
Each
Add to cart
Description:
SMART Digest Trypsin Kit, Soluble
Format:
SMART Digest Soluble Trypsin
Includes:
SMART Digest Soluble Trypsin, SMART Digest Buffer, Collection Plate
Price (USD)
818.83
Each
Add to cart

Obtain high quality analytical results from protein digests using Thermo Scientific™ SMART Digest™ Kits. A significant advance in sample preparation for biopharmaceutical protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.

Trypsin Protein Digestion

SMART Digest kits enable you to characterize and quantitate proteins faster, easier, and quicker than ever before. This version of the kit uses trypsin, which cleaves peptides on the C-terminal side of lysine and arginine amino acid residues.

Easy and simple
  • Significantly increased speed of sample processing
  • Simple process
  • Easily automate entire protocol for high-throughput processing
Reliable Results
  • Increased reproducibility over existing protocols
  • Higher sensitivity
  • Higher data confidence
Significantly Reduces Sample Preparation Time

Typically it takes less than 60 minutes (dependent on sample complexity) to achieve full digestion, whereas an in-solution digest can take up to 24 hours.

Cleaner Samples

Reduction in the number of chemicals used and the immobilized trypsin design helps mitigate against chemically induced post translational modifications (PTMs) and autolysis. This results in results which are easier to interpret.

Couple with SOLAμ SPE Plates for Desalting

When used with the SMART Digest kits for desalting, Thermo Scientific™ SOLAμ™ SPE solid phase extraction plates provide a fast, easy-to-use, robust, and easily automatable sample preparation solution for analysis of biomolecules.

Specifications
TypeProtein Digestion
DescriptionSMART Digest Trypsin Kit, Soluble
FormatSMART Digest Soluble Trypsin
EnzymeTrypsin
CleavageC-terminal side of lysine and arginine amino acids
CaptureNone
Product LineSMART Digest
For Use With (Application)Peptide Quantitation, Peptide Mapping
IncludesSMART Digest Soluble Trypsin, SMART Digest Buffer, Collection Plate
Unit SizeEach

Frequently asked questions (FAQs)

Can I use the SMART Digest Soluble Trypsin Kit with filter-aided sample preparation (FASP)?

Yes, we recommend the following protocol, which can be optimized as needed:
1. Rinse a Fisher Scientific Vivacon 500, 30kDa MWCO filter (Cat. No. 14558347) to remove trace amounts of glycerin using a fill volume of water. Decant the filtrate and collection vessel. The filter is now ready for use. If you do not want to use the Vivacon filter immediately, then store the pre-rinsed device in the refrigerator with water or buffer covering the membrane surface. Do not allow the membrane to dry out.
2. Dilute sample water to a total volume of 150 µL on the filter.
3. Centrifuge 14,000 RPM for 15 minutes and discard the flow-through.
4. Add 150 µL water.
5. Centrifuge 14,000 RPM for 15 minutes and discard the flow-through.
6. Add 200 µL of SMART Digest buffer.
7. Change the collection vial and add 5 µL of the SMART Digest soluble enzyme to the filter.
8. Parafilm the filters to prevent evaporation of sample during digestion. Incubate the units in a heated bath at 70°C for 60 - 90 minutes.
9. Optional: As needed, add DTT (3 µL of 50 mM DTT), heat at 37°C and incubate for 30 minutes. Alkylate as needed (using 3 µL of 150 mM alkylating reagent).
10. After incubation, add 2 µL of 1% trifluoroacetic acid to the filter. Mix for 1 minute.
11. Centrifuge the filter units at 14,000 RPM for 10 min.
12. As needed, measure protein concentration using a Thermo Scientific NanoDrop Spectrophotometer, or similar procedure.

What is the best way to automate my workflow with the SMART Digest Soluble Trypsin Kit?

To automate the SMART Digest Soluble Trypsin Kit workflow, we recommend using deepwell plates with Sepra Seals, or PCR plates with iron-on seals in combination with an automated liquid handling platform. Any heating unit with a heated lid, capable of providing uniform heating at 70°C may be used.

Can I use chaotropes with the SMART Digest Soluble Trypsin Kit?

The kit is compatible with up to 1M urea, but incompatible with most concentrations of guanidine.

Can I use surfactants with the SMART Digest Soluble Trypsin Kit?

The kit is compatible with many surfactants including OGS, CHAPS, Nonidet, and NP-40.

Does digestion at high temperatures using the SMART Digest Soluble Trypsin Kit result in an increase in chemical modifications?

Thus far, studies have shown that by accelerating the reaction and minimizing the reaction time, fewer chemical modifications are observed in comparison to protocols utilizing elongated digestion times at lower temperatures. For more details, refer to the application note titled 'SMART Digest Compared to Classic In-Solution Digestion of Rituximab for In-Depth Peptide Mapping Characterization'.

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